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Fig. 3 | BMC Microbiology

Fig. 3

From: Differential gene content and gene expression for bacterial evolution and speciation of Shewanella in terms of biosynthesis of heme and heme-requiring proteins

Fig. 3

The chemical analyses of the extracellular compound. The samples and a PPIX standard (Sigma-Aldrich, St. Louis, MO) were dissolved in a solution containing 90% acetone and 10% 0.1 M NH4OH. a Electrospray ionization tandem mass spectrometry (ESI-MS/MS, precursor ion: 563.2-PPIX) analyses showing almost identical structure of the PPIX standard and the bacterial samples; b Ultraviolet-visible spectrograms of the bacterial extract (the hemH1 and hemH2 double mutant) and PPIX standard. The absorbance was measured at every 10 nm (nm) with a spectrometer (UV-1800, Mapada) and quartz cuvettes

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