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Table 3 Abundance bias ratios calculated for the 19 taxa after Illumina-amplicon sequencing of rpoB and 16S rRNA for the five mock communities

From: rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencing

   

Ratio of metabarcoding relative abundance of OTUs / expected relative abundance

   

Mock1

Mock3

Mock4

Mock5

 

16S copy number

Genome size (Mb)

rpoB

16S

rpoB

16S

rpoB

16S

rpoB

16S

E. mundtii

6

3.3

2.21

0.91

3.44

1.45

3.61

1.15

4.21

1.61

D. acidovorans

5

6.8

0.46

1.42

0.66

1.86

0.75

2.03

0.82

1.93

A. xyloxisidans

3

6.8

0.93

0.68

1.32

0.97

1.35

0.94

1.42

0.85

S. liquefaciens

7

5.3

0.43

0.34

0.71

0.82

0.79

0.91

0.87

0.97

A. faecalis

3

4.2

2.52

2.37

3.29

3.15

3.58

3.34

3.85

3.31

P. protegens

5

7.0

0.94

0.60

1.27

0.85

1.25

0.87

1.30

0.88

O. anthropi

4

5.2

2.89

1.22

3.33

1.59

3.67

1.64

3.64

1.68

Acidovorax sp

3

4.9

0.06

0.58

0.12

0.86

0.14

1.00

0.16

0.98

V. paradoxus

2

7.5

0.12

0.14

0.11

0.13

0.14

0.17

0.07

0.12

P. chlororaphis

5

7.3

1.29

0.40

1.61

0.57

1.72

0.56

1.74

0.60

P. putida

7

6.2

1.97

1.42

1.52

0.92

1.62

1.14

1.71

1.07

Paenibacillus sp

10

6.0

1.68

0.84

1.99

1.25

2.23

1.17

2.21

1.28

Sphingomonas sp

2

4.1

0.07

1.26

0.09

1.75

0.09

1.90

0.08

1.78

Sphingobacterium sp

7

6.9

0.09

1.40

0.14

1.68

0.19

1.62

0.18

1.65

Brevundimonas sp.

2

3.4

1.13

0.41

1.21

0.60

1.30

0.59

1.25

0.57

Acinetobacter sp.

6

3.6

0.31

0.76

0.43

1.02

0.53

0.98

0.56

1.03

S. maltophilia

4

4.9

0.14

0.06

0.20

0.11

0.23

0.09

0.19

0.09

P. luminescens

7

5.7

1.56

0.97

6.66

4.01

5.60

2.97

10.76

3.63

X. nematophila

7

4.6

1.18

1.37

2.42

0.95

2.57

0.76

2.68

1.16