Table 3 Sequences of the primers used in this study
Primer | Primer sequences | Restriction site |
|---|---|---|
ΔsseK2-up-F | 5′-TCTAGAATAGAAGAGGCCCAAAGA-3’ | Xba I |
ΔsseK2-up-R | 5′-GGATCCATTTTTACACGCTTAAATTA-3’ | BamH I |
ΔsseK2-down-F | 5′-CTCGAG TCATGATAGCCTTGTTTTAC −3’ | Xho I |
ΔsseK2-down-R | 5′-GGTACC ACACGGCGCACTATTAGA-3’ | Kpn I |
sseK2- F | 5′-ACCACACTAACCAAAGCGCA-3’ |  |
sseK2 -R | 5′-GCAGAGAATAATGGACCACAT-3’ |  |
pBR -sseK2-F | 5′-GGATCCATGGCACGTTTTAATGCC-3’ | BamH I |
pBR -sseK2-R | 5′-CTCGACTTACCTCCAAGAACTGGCAG-3’ | Sal I |