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Table 2 Bacterial strains and plasmids used in this study

From: The impact of sseK2 deletion on Salmonella enterica serovar typhimurium virulence in vivo and in vitro

Strain or plasmid

Characteristics

Source or reference

Strains

 SL1344

Serovar Typhimurium, wild-type

[38]

 SL1344ΔsseK2

sseK2 deletion mutant

In this study

 χ7213

χ7213, containing plasmid of pREsseK2, Cmr

In this study

Plasmids

 

Laboratory stock

 pBluescriptIISK (+)

Phagemid cloning vector, oriCOLE1 oriF1(+) bla lacZa

In this study

 pBSKsseK2

pBluescriptIIKSt, sseK2

[39]

 pRE112

pGP704 suicide plasmid, pir dependent, oriT, oriV, sacB, Cmr

In this study

 pREΔsseK2

pRE112 dervative containing ssek2 fused in-frame, Cmr

Laboratory stock

 pBR322

oriColE1, AmprTcr

In this study

 pBR322-sseK2

pBR322 carrying the full sseK2gene (Ampr)

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