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Table 2 Bacterial strains and plasmids used in this study

From: The impact of sseK2 deletion on Salmonella enterica serovar typhimurium virulence in vivo and in vitro

Strain or plasmid Characteristics Source or reference
Strains
 SL1344 Serovar Typhimurium, wild-type [38]
 SL1344ΔsseK2 sseK2 deletion mutant In this study
 χ7213 χ7213, containing plasmid of pREsseK2, Cmr In this study
Plasmids   Laboratory stock
 pBluescriptIISK (+) Phagemid cloning vector, oriCOLE1 oriF1(+) bla lacZa In this study
 pBSKsseK2 pBluescriptIIKSt, sseK2 [39]
 pRE112 pGP704 suicide plasmid, pir dependent, oriT, oriV, sacB, Cmr In this study
 pREΔsseK2 pRE112 dervative containing ssek2 fused in-frame, Cmr Laboratory stock
 pBR322 oriColE1, AmprTcr In this study
 pBR322-sseK2 pBR322 carrying the full sseK2gene (Ampr)