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Fig. 5 | BMC Microbiology

Fig. 5

From: Universal membrane-labeling combined with expression of Katushka far-red fluorescent protein enables non-invasive dynamic and longitudinal quantitative 3D dual-color fluorescent imaging of multiple bacterial strains in mouse intestine

Fig. 5

3D in vivo imaging shows increased retention of colonizing bacteria in the early digestive tract compared with non-colonizing bacteria. Mice fed with fluorescently-labeled Nissle 1917 or Katushka-expressing TOP10 were imaged by FMT over time to track the progression of the bacteria (a). VOIs approximating the stomach through upper small intestine (1), lower small intestine (2), and cecum/colon (3) were placed on 3D reconstructed images. Dashed boxes represent VOIs placed towards the dorsal side of the mouse while solid boxes represent regions in the ventral side. (b) Fluorescences of individual VOIs were quantified and distribution of each bacteria over the 3 selected regions were determined. The Nissle 1917 shows preferential retention in regions 1 and 2 over the 24 h period while the TOP10 progresses through the digestive tract with time and is primarily found in regions 2 and 3 after 24 h

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