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Table 3 Sensitivity and specificity of phenotypic ASTs and genotypic AMR predictions against the broth microdilution reference AST. Clinical breakpoints are applied as interpretive criteria for all ASTs

From: Determining antimicrobial susceptibility in Salmonella enterica serovar Typhimurium through whole genome sequencing: a comparison against multiple phenotypic susceptibility testing methods

Antimicrobial Sensitivity (95% CI) Specificity (95% CI)
Disk diffusion Agar dilution Genotyping Disk diffusion Agar dilution Genotyping
Ampicillin 0.95 (0.85–0.99) 0.95 (0.85–0.99) 0.96 (0.88–1) 1 (0.92–1) 1 (0.92–1) 0.93 (0.82–0.99)
Chloramphenicol 0.92 (0.8–0.98) 0.71 (0.57–0.83) 0.94 (0.83–0.99) 0.98 (0.9–1) 0.98 (0.9–1) 0.94 (0.84–0.99)
Gentamicin 0.71 (0.29–0.96) 0.86 (0.42–1) 0 (0–0.41) 1 (0.96–1) 1 (0.96–1) 1 (0.96–1)
Nalidixic Acid 0.82 (0.63–0.94) 0.75 (0.55–0.89) 0.82 (0.63–0.94) 1 (0.95–1) 0.96 (0.89–0.99) 1 (0.95–1)
Sulfamethoxazole 0.97 (0.9–1) 0.97 (0.9–1) 0.67 (0.54–0.78) 1 (0.89–1) 0.94 (0.8–0.99) 0.97 (0.84–1)
Streptomycin 0.96 (0.8–1) 0.38 (0.2–0.59) 0.92 (0.75–0.99) 0.59 (0.47–0.7) 1 (0.95–1) 0.43 (0.32–0.55)
Trimethoprim-Sulfamethoxazole 1 (0.88–1) 0.93 (0.78–0.99) 0.97 (0.83–1) 1 (0.95–1) 0.97 (0.9–1) 0.96 (0.88–0.99)
Overall 0.94 (0.9–0.96) 0.83 (0.78–0.87) 0.84 (0.79–0.88) 0.93 (0.9–0.95) 0.98 (0.97–0.99) 0.88 (0.85–0.91)