Use of GC–MS based metabolic fingerprinting for fast exploration of fungicide modes of action

BMC Microbiology

Table 1 EC₅₀ values of different mode of action fungicides to B. cinerea SP2–6

Mode of action group	Fungicides	CAS	EC₅₀ (μg/mL)	Concentrations used (μg/mL)
β-tubulins assembly	Carbendazim	10,605–21-7	0.04	0, 0.005, 0.013, 0.05, 0.1, 0.2
β-tubulins assembly	Thiophanatemethyl	23,564–05-8	0.36	0, 0.2,0.3, 0.4, 0.6, 0.9
Respiration	Boscalid	188,425–85-6	0.08	0, 0.02, 0.05, 0.1, 0.5, 1
	Kresoxim-methyl	143,390–89-0	0.13	0, 0.02, 0.04, 0.08, 0.78, 1.56
	Fluazinam	79,622–59-6	0.01	0, 0.001, 0.005, 0.05, 0.1, 0.2
Mitochondrial function	Cyprodinil	121,552–61-2	0.47	0, 0.15, 0.3, 1, 3, 15
Mitochondrial function	Pyrimethanil	53,112–28-0	0.68	0, 0.01, 0.5, 1, 2, 5
Signal transduction	Fludioxonil	131,341–86-1	0.02	0, 0.005, 0.01, 0.02, 0.04, 0.05
Signal transduction	Procymidone	32,809–16-8	0.39	0, 0.23, 0.45, 0.6, 0.8, 0.9
Membrane sterol biosynthesis	Imazalil	35,554–44-0	4.64	0, 1, 2.5, 5, 10, 15
Membrane sterol biosynthesis	Fenhexamid	126,833–17-8	0.41	0, 0.1, 0.25, 0.5, 1, 5
Multi site action	Chlorothalonil	1897-45-6	5.41	0, 0.1, 0.5, 1, 10, 50
Multi site action	Thiram	137–26-8	1.59	0, 0.39, 0.78, 1.56, 3.13, 6.25
Unknown	SYP-14288		0.004	0, 0.001, 0.003, 0.005, 0.01, 0.05

Note: The EC₅₀ values of fungicides against B. cinerea SP2–6 were measured based on the mycelial growth rate method on PDA plates. Each combination of isolate and fungicide concentration was represented by 6 replicate plates, and the experiments were performed 3 times

ISSN: 1471-2180