Carbohydrate metabolic systems present on genomic islands are lost and gained in Vibrio parahaemolyticus

BMC Microbiology

Table 2 Primers used in this study

Primer name and use	Sequence (5′-3′)^a	Melting temp (°C)
SOE PCR
VP0065 SOEA	TCTAGAAACCGTAGCGCTAACCACTA	63
VP0065 SOEB	AATGTCACTTCCGCACATGG	59
VP0065 SOEC	CCATGTGCGGAAGTGACATTTGCAATGATGGATAACGGCG	59
VP0065 SOED	GAGCTCCAGGCCCCAATTTTGAGACC	60
VP0065 SOEFF	GTGTGGTGAGTCATTGGCTC	55
VP0065 SOEFR	GCCAATGCGTACGACAAAGA	50
SGLT SOEA	TCTAGACTTCTACCCTTTGAACTTGCAG	58
SGLT SOEB	GACATAAATGGCGAAGACC	51
SGLT SOEC	GGTCTTCGCCATTTATGTCATCGCTGCTTACGGCATAAT	66
SGLT SOED	GAGCTCGGTTTGTATGCACCACCCAC	63
SGLT SOEFF	GGTGAAAACCACCAAACCTG	55
SGLT SOEFR	TGAGGATGGGCGTTCATAAT	54
Quantitative real-time PCR (qPCR) primers
VP0065 F	ACCGGGTGTAAACAACCCAA	60
VP0065R	TACCGCCACAAGCTACAACC	60
VP1708F	CACGCCAGTAGAAGGTGACA	60
VP1708R	GTTGCCTAGGAACGCAGAAC	59
VPA0083F	GCAAGGCAGTGAAATTGGCA	60
VPA0083R	ACGTTACGCTGTTGGCAGTA	60
VPA1708F	CAGCCGCTGAAATCACCTTC	55
VPA1708R	TTTCTTGGCACGCTTTGACG	50
16S F	ACGGCCTGGGGAGTACGGTC	60
16S R	TTGCGCTCGTTGCGGGACTT	60
Complementation primers
SGLTforward	TCTAGAGGATGATCCCCGTAATTTCC	58
SGLTreverse	GAGCTCATTTGGCAGCAAGGTTGAATC	61

ISSN: 1471-2180