Skip to main content

Table 4 Spontaneous mutation obtained from 0.2 μg/mL of rifampin in rpoB gene of 250 rifampin-resistant R. anatipestifer

From: Rifampin resistance and its fitness cost in Riemerella anatipestifer

Codon mutation

Nucleic acid change

No. of strains

Frenquency (%)

RRDRs

MIC (μg/mL)

MIC fold change

Q478R

CAG → CGG

24

9.6

I

128

32,000

Q478K

CAG → AAG

3

1.2

I

128

32,000

D481V

GAT→GTT

19

7.6

I

128

32,000

D481Y

GAT→TAT

6

2.4

I

8

2000

S487Y

TCT → TAT

2

0.8

I

128

32,000

S487F

TCA → TTA

14

5.6

I

128

32,000

H491Y

CAT→TAT

30

12

I

128

32,000

H491D

CAT→GAT

2

0.8

I

64

16,000

H491R

CAT→CGT

1

0.4

I

128

32,000

R494K

AGA → AAA

110

44

I

128

32,000

S496 L

TCA → TTA

36

14.4

I

128

32,000

I537N

ATT → AAT

1

0.4

II

0.5

125

S539F

TCT → TTT

2

0.8

 

0.5

125

485::TAA

::TAA

1

0.4

 

128

32,000

S496 L+  535::G

::G

1

0.4

 

128

32,000

Total

 

252a

100.8a

   
  1. a.the reason why strain numbers are greater than the total number of mutants (250) and the frequencies are greater than 1 is that the presence of two double mutant strains
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us