Skip to main content

Table 1 RNA yield and quality for three different RNA extraction methodsa

From: Comparison of the efficiency of different cell lysis methods and different commercial methods for RNA extraction from Candida albicans stored in RNAlater

Storage medium

RNA extraction method

RNA yield and qualityb

NanoDrop

Fragment Analyzer

 

RNA yield (μg)

A260/A280c

RNA yield (μg)

RQNd

TSB-Ge

RNeasy Mini Kit

2.68 ± 0.04

1.93 ± 0.04

2.87 ± 0.13

10.00 ± 0.00

TSB-Ge

RiboPure Yeast Kit

6.01 ± 1.27

2.02 ± 0.03

4.12 ± 0.57

9.50 ± 0.02

TSB-Ge

NucliSENS easyMAG

1.86 ± 0.59

2.14 ± 0.08

1.70 ± 0.32

9.87 ± 0.12

RNAlater

RNeasy Mini Kit

2.43 ± 0.11

1.84 ± 0.06

2.61 ± 0.15

10.00 ± 0.00

RNAlater

RiboPure Yeast Kit

7.10 ± 0.22

1.98 ± 0.02

5.82 ± 0.03

7.77 ± 0.75

RNAlater

NucliSENS easyMAG

2.32 ± 0.20

2.01 ± 0.07

2.17 ± 0.06

10.00 ± 0.00

  1. aAll extraction methods after horizontal bead beating in cognate lysis buffer
  2. bResults are means and standard deviations of six independent extractions
  3. cQuality for NanoDrop is shown as the A260/A280 ratio. A value of ~ 2.0 is generally accepted as indicating that RNA is free of proteins
  4. dQuality for Fragment Analyzer is shown as the RQN value. Reported on a scale of 1 to 10, with higher values indicating a better quality of total RNA. Values above 7 are considered to represent high quality and non-degraded RNA
  5. eTryptic Soy Broth + 15% glycerol
Back to article page

BMC Microbiology

ISSN: 1471-2180

Contact us