Fig. 4
Analysis of the NK production mediated by different triple-promoter systems. a Fibrinolytic activities of NK in the supernatant. b SDS-PAGE analysis of the culture supernatant. Recombinant strains promoted by different triple-promoters were cultured in the TB medium for 36 h, and then cells and the supernatant culture were separated by centrifugation. Lane 1–8: PP43-PP43-PHpaII, PP43-PHpaII-PHpaII, PHpaII-PP43-PP43, PP43-PHpaII-PP43, PP43-PP43-PP43, PHpaII-PHpaII-PP43, PHpaII-PP43-PHpaII, and PHpaII-PHpaII-PHpaII; Lane M: standard marker proteins. The arrow indicates NK bands