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Table 2 pDCs stimulatory activity of LAB strains with varied GC contents in genome DNA

From: Characterization of genomic DNA of lactic acid bacteria for activation of plasmacytoid dendritic cells

Species

Strains

 

IFN-α (pg/mL)*

CpG frequency (per kb)**

GC (%)

High***

Lactococcus lactis subsp. lactis

LC-Plasma

 

404.4

22.3

35.3

2/3

JCM 20101

 

389.8

ATCC 15577

 

12.2

  

mean ± S.D.

271.0 ± 222.4

   

Pediococcus pentosaceus

JCM 2026

 

84.0

28.4

37.4

5/19

JCM 20109

 

353.3

JCM 20314

 

102.1

JCM 20459

 

5.5

NBRC 3182

 

59.1

NBRC 3891

 

15.8

NBRC 3892

 

116.4

NBRC 3893

 

32.2

NBRC 3894

 

46.0

NBRC 12229

 

18.7

NBRC 12230

 

7.9

NBRC 12232

 

14.8

NBRC 12318

 

59.8

NBRC 101982

 

404.4

NBRC 101983

 

5.6

NBRC 101984

 

5.9

NBRC 101985

 

5.8

NBRC 101986

 

172.3

NBRC 101987

 

34.3

  

mean ± S.D.

81.3 ± 114.5

   

Lenconostoc mesenteroides

NBRC 3349

 

3.2

27.1

37.7

2/10

NBRC 3426

 

57.3

NBRC 3832

 

353.5

NBRC 12060

 

4.2

NBRC 100495

 

5.7

NBRC 100496

 

8.1

NBRC 102497

 

36.0

NBRC 102480

 

74.2

NBRC 102481

 

167.0

NBRC 107766

 

4.4

  

mean ± S.D.

71.4 ± 111.6

Lactobacillus low GC (< 40%)

Lactobacillus acidophilus

JCM 1132

 

11.7

15.4

34.7

0/5

Lactobacillus plantarum

JCM 1551

 

16.7

4.6

39.1

JCM 6651

 

55.2

JCM 8341

 

4.0

JCM 20110

 

9.8

  

mean ± S.D.

19.5 ± 20.5

  

Lactobacillus high GC (> 40%)

Lactobacillus casei

ATCC 393

 

5.6

1.5

46.6

0/4

Lactobacillus rhamnosus

ATCC 53103

 

3.9

4.0

47.0

Lactobacillus fermentum

NBRC 3959

 

4.4

1.9

51.5

NBRC 3961

 

5.2

  

mean ± S.D.

4.8 ± 0.8

  
  1. *IFN-α producing activity of whole cells of LAB strains
  2. **CpG frequency in the low G + C region of the genomes. Strains used for calculation are shown in the Methods
  3. ***Number of high (> 100 pg/mL)-IFN-α-producing strains per tested strains
  4. The origin and cultivation methods of the strains are shown in the Methods