Fig. 1

Growth of the triple knockouts in minimal medium. (a) Bacteria were grown overnight in TGP medium, with 1 mM Pi as a phosphorus source. a, wild-type strain MG1655; b, strain RI57 (ΔpitA Δpst ΔugpphnE⁺ - spontaneous phnE⁺ revertant); c, strain RS07 (phnE⁺3 Δ); d, strain RS04 (pitA⁺3 Δ); e, strain RS05 (ugp⁺3 Δ); f, strain RS06 (pst⁺3 Δ). (b) Growth curve of strain RS07 (phnE⁺ 3 Δ) in TGP. Bacteria were diluted to an OD ₆₀₀ of 0.1 and grown for 24 h in medium TGP (1 mM Pi). (red square), wild-type strain MG1655; (blue square), RS07 (phnE⁺3 Δ). (c) Growth of strain RS05 ()(ugp⁺ 3 Δ) with Pi or glycerol phosphate as phosphorus sources. Bacteria were grown overnight in TGP (1 mM Pi) or TGG3P (1 mM G3P). WT, wild-type strain MG1655. Red crossed stripes, TGP medium; blue cross stripes, TGG3P medium. (d) Growth of the triple and quadruple mutants RS07 (phn⁺3 Δ) and RS08 (phn⁺ ΔpitA ΔpitB Δpst Δugp) in TGP medium; WT, strain MG1655. Each bar represents the mean ± S.E.M. of three independent cultures