Skip to main content

Advertisement

Table 4 Proteins associated with copper response in P. aeruginosa PAO1 whole cell lysate. Protein samples from the copper stressed PAO1 were compared against the control group (+/− 1.5 –fold abundance change, p < 0.01)

From: Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress

Protein Fold change (+/−) p-value Peptides Product Description Subcellular Localization (localization [confidence code])* Product Name Confidence
PA3661 63.0 < 0.0001 2 Hypothetical protein Unknown [Class 3] Class 4
PA2505 25.1 < 0.0001 10 Tyrosine porin OpdT Outer Membrane [Class 2]; Outer Membrane [Class 3]; Outer Membrane Vesicle [Class 1] Class 1
PA4714 16.2 0.002 5 Conserved hypothetical protein Unknown (This protein may have multiple localization sites) [Class 3]; Unknown (This protein may have multiple localization sites) [Class 3] Class 4
PA2064 10.8 < 0.0001 6 Copper resistance protein B precursor Outer Membrane [Class 3]; Outer Membrane Vesicle [Class 1] Class 2
PA2405 10.3 0.003 1 FpvJ Unknown [Class 3] Class 1
PA3412 10.2 0.005 3 Hypothetical protein Unknown [Class 3] Class 4
PA2807 10.1 0.004 4 Hypothetical protein Unknown [Class 3] Class 4
PA4224 6.6 0.002 6 Pyochelin biosynthetic protein PchG Cytoplasmic [Class 3] Class 1
PA3445 6.2 0.002 3 Conserved hypothetical protein Unknown (This protein may have multiple localization sites) [Class 3]; Unknown (This protein may have multiple localization sites) [Class 3] Class 4
PA4884 4.9 0.004 4 Hypothetical protein Periplasmic [Class 3] Class 4
PA2065 4.4 < 0.0001 8 Copper resistance protein A precursor Periplasmic [Class 2]; Periplasmic [Class 3]; Outer Membrane Vesicle [Class 1] Class 2
PA3920 4.0 0.003 2 Probable metal transporting P-type ATPase Cytoplasmic Membrane [Class 3] Class 3
PA3182 3.0 0.008 2 6-phosphogluconolactonase Unknown [Class 3] Class 1
PA0283 2.8 0.009 10 Sulfate-binding protein precursor Periplasmic [Class 2]; Periplasmic [Class 3] Class 2
PA3450 2.8 0.001 6 Probable antioxidant protein Cytoplasmic [Class 3] Class 3
PA0346 2.5 0.004 6 Hypothetical protein Unknown [Class 3] Class 4
PA4923 2.3 0.010 2 Conserved hypothetical protein Unknown [Class 3] Class 4
PA3165 2.0 0.007 5 Histidinol-phosphate aminotransferase Cytoplasmic [Class 3] Class 2
PA5215 1.9 0.006 1 Glycine-cleavage system protein T1 Cytoplasmic [Class 3] Class 2
PA5335 1.7 0.005 1 Conserved hypothetical protein Cytoplasmic [Class 3] Class 4
PA4240 1.7 0.009 3 30S ribosomal protein S11 Cytoplasmic [Class 3]; Outer Membrane Vesicle [Class 1] Class 2
PA0473 1.7 0.006 2 Probable glutathione S-transferase Cytoplasmic [Class 3] Class 3
PA5188 1.7 0.010 2 Probable 3-hydroxyacyl-CoA dehydrogenase Cytoplasmic [Class 3] Class 3
PA3029 1.6 0.007 5 Molybdopterin biosynthetic protein B2 Cytoplasmic [Class 3] Class 2
PA4110 1.6 0.010 5 Beta-lactamase precursor (AmpC) Periplasmic [Class 1]; Periplasmic [Class 3] Class 1
PA1024 1.6 < 0.0001 1 2-Nitropropane Dioxygenase Cytoplasmic [Class 3] Class 1
PA3896 1.6 0.001 2 Probable 2-hydroxyacid dehydrogenase Cytoplasmic [Class 2]; Cytoplasmic [Class 3] Class 3
PA4947 1.5 0.007 1 N-acetylmuramoyl-L-alanine amidase Unknown [Class 3] Class 2
PA1151 1.5 0.005 3 Pyocin S2 immunity protein Cytoplasmic [Class 3] Class 1
PA3243 1.5 0.001 3 Cell division inhibitor MinC Cytoplasmic [Class 3] Class 2
PA2572 −1.6 0.008 1 Probable two-component response regulator Cytoplasmic [Class 3] Class 3
PA0833 −1.6 0.008 2 Hypothetical protein Outer Membrane [Class 3]; Outer Membrane Vesicle [Class 1] Class 4
PA2476 −1.7 0.008 3 Thiol:disulfide interchange protein DsbG Periplasmic [Class 3] Class 2
PA5209 −1.9 0.001 2 Hypothetical protein Unknown (This protein may have multiple localization sites) [Class 3]; Unknown (This protein may have multiple localization sites) [Class 3] Class 4
PA5562 −1.9 0.002 5 Chromosome partitioning protein Spo0J Cytoplasmic [Class 3] Class 2
PA0265 −2.0 0.004 3 Succinate-semialdehyde dehydrogenase Cytoplasmic [Class 3] Class 2
PA5554 −2.0 < 0.0001 15 ATP synthase beta chain Cytoplasmic [Class 3]; Outer Membrane Vesicle [Class 1] Class 2
PA2259 −2.1 0.006 1 Transcriptional regulator PtxS Cytoplasmic [Class 3] Class 1
PA4317 −2.2 0.006 1 Hypothetical protein Cytoplasmic Membrane [Class 3] Class 4
PA4764 −2.3 0.008 2 Ferric uptake regulation protein Cytoplasmic [Class 1]; Cytoplasmic [Class 3] Class 1
PA1580 −2.3 0.009 5 Citrate synthase Cytoplasmic [Class 3] Class 1
PA4360 −2.4 0.008 2 Hypothetical protein Unknown [Class 3]; Outer Membrane Vesicle [Class 1] Class 4
PA1608 −2.5 0.008 2 Probable chemotaxis transducer Cytoplasmic Membrane [Class 3]; Outer Membrane [Class 2] Class 3
PA3214 −2.7 0.002 2 Hypothetical protein Unknown [Class 3] Class 4
PA0762 −2.7 0.007 2 Sigma factor AlgU Cytoplasmic [Class 3] Class 1
PA0122 −2.8 0.003 4 rahU Extracellular [Class 3] Class 1
PA4310 −2.9 0.004 3 Chemotactic transducer PctB Cytoplasmic Membrane [Class 3] Class 1
PA2654 −3.2 0.008 6 Probable chemotaxis transducer Cytoplasmic Membrane [Class 3] Class 3
PA2815 −4.2 0.006 4 Probable acyl-CoA dehydrogenase Cytoplasmic Membrane [Class 3]; Outer Membrane Vesicle [Class 1] Class 3
PA4876 −4.7 0.003 2 Osmotically inducible lipoprotein OsmE Unknown [Class 3]; Outer Membrane Vesicle [Class 1] Class 2
PA1324 −6.1 0.003 5 Hypothetical protein Unknown [Class 3] Class 4
PA3691 −6.2 0.009 8 Hypothetical protein Unknown [Class 3]; Outer Membrane Vesicle [Class 1] Class 4
PA3110 −16.7 < 0.0001 1 Hypothetical protein Unknown [Class 3] Class 4
  1. *Extracted from PGD. Class 1: Subcellular localization experimentally demonstrated in Pseudomonas aeruginosa. Class 2: Subcellular localization of a similar gene experimentally demonstrated in another organism OR to a paralog experimentally demonstrated in the same organism. BLAST expect value of 10e-10 for query within 80–120% of subject length. Class 3: Subcellular localization computationally predicted by PSORTb [44]. Bold indicates first time evidence of protein translation in PAO1