Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri

Kalindamar, Safak; Lu, Jingjun; Abdelhamed, Hossam; Tekedar, Hasan C.; Lawrence, Mark L.; Karsi, Attila

doi:10.1186/s12866-019-1429-3

BMC Microbiology

Table 1 Unique E. ictaluri genes with transposon insertion

From: Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri

Mutant	Locus	Product	E-value^a	Frequency^b	TA Frequency
Eis001	NT01EI_1281	NAD-dependent malic enzyme (NAD-ME)	2.00E-72		64
Eis002	NT01EI_1721	PTS system, mannose/fructose/sorbose family, IIB component	2.00E-106		32
Eis004	NT01EI_0182	Hypothetical protein	7.00E-57		26
Eis006	NT01EI_0085	ATP-dependent DNA helicase Rep	2.00E-114		46
Eis009	NT01EI_1236	Coproporphyrinogen III oxidase, aerobic	6.00E-32		24
Eis011	NT01EI_3690	ABC transporter, periplasmic amino acid binding protein	1.00E-74		50
Eis013	NT01EI_2795	Translocator protein, LysE family	2.00E-115		36
Eis018	NT01EI_0377	Aspartate ammonia-lyase	7.00E-145		59
Eis024	NT01EI_3505	Dihydrouridine synthase Dus	1.00E-129		59
Eis027	NT01EI_0408	tRNA delta(2)-isopentenylpyrophosphate transferase	5.00E-94		22
Eis028	NT01EI_0277	Transposase, IS4 family protein	1.00E-73	4	17
Eis029	NT01EI_2683	Membrane protein	2.00E-88		27
Eis033	pEI2_p2	Putative Rep protein	2.00E-123	5	31
Eis035	pEI1_p4	Putative RNA one modulator protein	9.00E-19		12
Eis037	NT01EI_2355	eseJ	3.00E-129	2	88
Eis038	NT01EI_1334	eseM	5.00E-67	2	91
Eis039	NT01EI_3177	FeS assembly protein IscX	3.00E-38		7
Eis041	NT01EI_0943	eseC	8.00E-116		53
Eis048	NT01EI_3148	Hypothetical protein	4.00E-80		20
Eis055	NT01EI_2314	Prophage lambda integrase	8.00E-128		56
Eis059	NT01EI_1941	Hypothetical protein	3.00E-70		8
Eis065	NT01EI_2281	Excinuclease ABC subunit C	3.00E-48		64
Eis068	NT01EI_0448	Polyprenyl synthetase	1.00E-115		37
Eis080	NT01EI_0981	Hypothetical protein	8.00E-129	9	58
Eis086	NT01EI_1237	N-acetylmuramoyl-L-alanine amidase AmiA	3.00E-67		25
Eis107	NT01EI_0475	DEAD box containing helicase	1.00E-112		51
Eis110	NT01EI_1332	eseL	4.00E-26	2	73
Eis131	NT01EI_2157	Hypothetical protein	4.00E-66		37
Eis152	NT01EI_0224	Transporter, major facilitator family	1.00E-16	4	47
Eis154	NT01EI_3522	Selenate reductase, FAD-binding subunit	2.00E-73		23
Eis155	NT01EI_0725	Transcriptional regulator FruR	3.00E-134		43
Eis156	NT01EI_2381	Ribonuclease, RNaseE/RNaseG family	2.00E-04		72
Eis157	NT01EI_0144	Twin-arginine translocation protein subunit TatB	8.00E-07		13
Eis158	NT01EI_0022	Sulfurtransferase, TusA	8.00E-46		10
Eis171	NT01EI_3723	Magnesium-translocating P-type ATPase	0		71
Eis172	NT01EI_3786	Hypothetical protein	1.00E-26		24
Eis173	NT01EI_3265	Acyltransferase/AMP-dependent synthetase and ligase family	0	2	54
Eis174	NT01EI_3721	Hypothetical protein	3.00E-25		7
Eis175	NT01EI_3774	IS1 transposase	7.00E-93		19
Eis176	NT01EI_0962	esaT	5.00E-129		27
Eis180	NT01EI_3103	UPF0126 domain protein	7.00E-12		16
Eis183	NT01EI_3105	Chloride transporter, chloride channel (ClC) family	6.00E-166		53
Eis184	NT01EI_0419	RNA methyltransferase, TrmH family, group 3	9.00E-27		21
Eis185	NT01EI_3386	TRAP transporter, DctM subunit	7.00E-85		47
Eis192	NT01EI_3147	Hypothetical protein	0	2	103
Eis194	NT01EI_1981	Universal stress protein A uspA	9.00E-86	3	20
Eis195	NT01EI_0376	Anaerobic C4-dicarboxylate transporter DcuA	2.00E-159	11	47
Eis207	NT01EI_1817	Spermidine/putrescine transport system permease protein PotB	4.00E-132		22
Eis210	NT01EI_0800	Prolipoprotein diacylglyceryl transferase	5.00E-79		36
Eis220	NT01EI_2076	Hypothetical protein	6.00E-20		5
Eis222	NT01EI_0768	Hypoxanthine phosphoribosyltransferase	5.00E-127		24
Eis223	NT01EI_1086	Extracellular solute-binding protein, family 5	2.00E-142		48
Eis230	NT01EI_3769	Phosphoglycerate transporter family protein	0		49
Eis232	NT01EI_2010	Hypothetical protein	2.00E-136	3	58
Eis233	NT01EI_2530	Putative permease, membrane region	4.00E-81		65
Eis235	NT01EI_3289	Diaminopimelate decarboxylase	9.00E-36		38

^aBlastx E-value
^bpMAR2xT7 insertion frequency

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