Fig. 1

Overview of experimental design. Porphyromonas gingivalis ATCC 33277 strain was maintained on blood agar plates and grown in modified Brain Heart Infusion (BHI) medium. After 24 h, optical density was measured, and a pure culture containing 10⁸ colony forming units per milliliter (CFU/mL) was set. Two culture conditions were then prepared: Test cells, depositing P. gingivalis cells in presence of Hydroxyapatite (HA) disc, and Control cells, depositing P. gingivalis cells in the wells without HA discs. After 96 h of incubation of multi-well plates under anaerobic conditions, free floating P. gingivalis cells from both test and control condition were harvested, examined by CLSM, processed and total RNA extracted and purified. Agilent Oligo Microarrays 8x15K (074976) for P. gingivalis ATCC 33277 were used for hybridizations, (this slides also contents probes against the whole genome of P. gingivalis W83), and RT-qPCR analyses were performed to confirm the results. The experiments were repeated three times and each experimental condition was pooled into the three biological replicates and processed for the transcriptomic analysis. (Images for Fig. 1 were taken from https://smart.servier.com/ under a creative commons licence)