Fig. 8
Immunization with Mi reduces the Mtb burden in the lungs and spleen. Mice (4 animals/group) were immunized with Mi (1 × 106 bacilli/animal). The control groups were inoculated with BCG (positive control) and placebo PBS (negative control). After 30 days, animals were aerosol challenged with live Mtb. After 45 days of infection, animals were sacrificed and protective efficacy of immunization was determined by calculating bacterial burden in (a) spleen; (b) lungs by colony forming units (CFUs). Data represented as CFU/gm of tissue (log10) are from triplicate plates. *p < 0.05, **p < 0.005