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Fig. 2 | BMC Microbiology

Fig. 2

From: The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens

Fig. 2

Location and sequences of primers used in the LAMP assays. The positions of the LAMP primers are shown relative to the sodA gene fragment of Enterococcus hirae (accession no. CP003504.1). Right and left arrows indicate sense and complementary sequences. Green boxes: indicate the outer primers F3 and B3 (product size 248 bp); Blue boxes: indicate forward inner primer FIP (F1c + F2); Grey boxes: indicate backward inner primer BIP (B1c + B2); Yellow boxes: indicate Loop primers LF and LB. Red font indicates the location and sequence of LAMP product (139 bp). Blue font indicates the location of species-specific primers used in standard PCR (product size 187 bp)

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