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Table 5 HPLC-MS analyses of the active desert actinobacterial strain

From: Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling

Strains Thin Layer Chromatography (TLC) Profile HPLC-Retention time (tR/min) (+) and (−)-ESI-MS: (m/z) Molecular Weights Activity against MRSA Suggested Streptomyces metabolites from AntiBase 2017
(Additional files 15 and 20)
UV Visualization Staining with Anisaldehyde/H2SO4
AFD2 254 nm 4 bands 2green,
1purple,
2yellow
6.38
11.03
12.57
113 [M + H]+, 111[M-H]
208 [M + H]+, 206 [M-H]
137 [M + H]+, 135 [M-H]
112
207
136
17 mm zone of inhibition against MRSA A5;32 mm zone of inhibition against MRSA A11 MW = 112: Emimycin, 3,5-Dimethyl-3-oxol-2-on, Uracil, Enimycin.
MW = 207: Triacsin C, Triacsin D, Streptazolin, 2-(2-Hydroxypropionyl)acetanilide, Aeruginol, 7-Deoxyechinosporin.
MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.
366 nm 2 bands
AFD4 254 nm 3 bands 2 purple bands, 1 light blue band, 1 yellow band, 1 green band 10.23
12.22
12.60
211 [M + H]+, 209 [M-H]
146 [M + H]+
137 [M + H]+, 135 [M-H]
210
145
136
9 mm zone of inhibition against MRSA A11;18 mm zone of inhibition against MRSA A5 MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline
MW = 146: Indole-3-carbaldehyde
MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.
366 nm 2 bands
AFD7 254 nm 4 bands 2 green bands, 2 yellow bands, 1 blue band 3.83
4.50
5.77
7.10
10.24
11.07
11.38
11.91
13.16
15.05
16.72
153 [M + H]+, 151 [M-H]
202 [M + H]+
261 [M + H]+, 259 [M-H]
115 [M + H]+, 113 [M-H]
211 [M + H]+
145 [M + H]+, 143 [M-H]
424 [M + H]+, 422 [M-H]
407 [M + H]+, 405 [M-H]
494 [M + H]+
231 [M + H]+, 229 [M-H]
390 [M + H]+, 388 [M-H]
223 [M + H]+, 245 [M + Na]+
152
201
260
114
210
144
423
406
493
230
389
222
20 mm zone of inhibition against MRSA A11; 25 mm zone of inhibition against MRSA A7 MW = 152: Guanazolo, Orsellin-aldehyde, MY3–469, Xanthine.
MW = 260: Cyclo(L-Tyr-L-Pro), VibrindoleA
MW = 114: 4-Hydroxy-5-methyl-furan-3-one
MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline
MW = 144: Pentenomycin I, 4-Epipentenomycin I
MW = 423: Fibrostatin B, A9145 A, 6-Amino-9 [(aminocarbonyl)amino]-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9- hexadeoxy-decafuranuronamide
MW = 389: Hatomamicin, SF-2809-I
366 nm 4 bands
AFD8 254 nm 4 bands 4 blue bands 9.07
13.42
14.94
15.58
153 [M + H]+, 151 [M-H]
257 [M + H]+, 255 [M-H]
225 [M + H]+
181 [M + H]+
152
256
224
180
12 mm zone of inhibition against MRSA A12;17 mm zone of inhibition against MRSA A1 MW = 152: o Hydroxyphenylacetic acid, BE-39907 A2
MW = 256: Phenacein, Albonoursin, N-Isobutyrylpyrrothine, 2,9-Dihydroxyphenazine-1-carboxylic acid
366 nm 3 bands
AFD10 254 nm 4 bands 3 yellow bands, 2 brown bands 9.90
12.60
15.61
19.42
211 [M + H]+
137 [M + H]+,135 [M-H]
401 [M + H]+, 399 [M-H]
475 [M + H]+, 473 [M-H]
210
136
400
474
17 mm zone of inhibition against MRSA A7; 20 mm zone of inhibition against MRSA A2 MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline.
MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.
MW = 400: Daunomycinol, Furaquinocin G, Daidzein-7-α-l-rhamnoside, Oxopropaline-E, 2 11-A.
MW = 474: N-Ethyloxytetracycline, KS-619-1.
366 nm 1 band
AFD12 254 nm 3 bands 1 dark yellow band, 1 pink band, 1 green band 3.70
4.36
6.43
12.50
16.06
-
160 [M + H]+,158 [M-H]
110 [M-H]
205 [M-H]
137 [M + H]+, 135 [M-H]
211 [M + H]+
-
159
111
206
136
210
11 mm zone of inhibition against MRSA A7; 15 mm zone of inhibition against MRSA A5 MW = 159: 4-(Hydroxymethyl)quinolone, Quinazolinamine
MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.
MW = 210: Nikkomycin E, (4R)-Thiolactomycin, Cyanomycin, 2-Amino-4-hydroxy-3-methyl-4-(3-pyridyl)butanoic acid.
366 nm 2 bands
AFD26 254 nm 4 bands 1 brown bands, 1 purple band, 1 light blue band, 1 greenish blue band .48
6.32
6.38
9.49
10.85
12.23
17.43
1052 [M + H]+??
113 [M + H]+, 111 [M-H]
164 [M + H]+, 162 [M-H]
164 [M + H]+, 162 [M-H]
164 [M + H]+, 162 [M-H]
317 [M + H]+
391 [M + H]+
112
163
163
163
316
390
10 mm zone of inhibition against MRSA A1; 15 mm zone of inhibition against MRSA A7 MW = 112: Emimycin, 3,5-Dimethyl-3-oxol-2-on, Uracil, Enimycin.
MW = 163: L-β-(3-Hydroxyureido)-alanine, Homoalanosine, β-Phenylethylacetamide, 2-N-Hydroxy-3,4-dihydroisoquinoline-1-one, Streptazone D, 2-Amino-4-hydroxy-pterin, 6-Hydroxy-isatin, Dihydroabikoviromycin, 3-Nitro-1H-indazole, Kitasatodine
MW = 316: 17927-D, Boxazomycin A, 1 Isobutyroxymethyl cyclohex-1(6)-ene-2,3,4,5-tetrol-2-isobutyrate.
MW = 390: SF-2330, Arizonin C3.
366 nm 2 bands
  1. For details, see Supplementary Information (Figure S3-S20)