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Table 5 HPLC-MS analyses of the active desert actinobacterial strain

From: Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling

Strains Thin Layer Chromatography (TLC) Profile HPLC-Retention time (tR/min) (+) and (−)-ESI-MS: (m/z) Molecular Weights Activity against MRSA Suggested Streptomyces metabolites from AntiBase 2017 (Additional files 15 and 20)
UV Visualization Staining with Anisaldehyde/H2SO4
AFD2 254 nm 4 bands 2green, 1purple, 2yellow 6.38 11.03 12.57 113 [M + H]+, 111[M-H] 208 [M + H]+, 206 [M-H] 137 [M + H]+, 135 [M-H] 112 207 136 17 mm zone of inhibition against MRSA A5;32 mm zone of inhibition against MRSA A11 MW = 112: Emimycin, 3,5-Dimethyl-3-oxol-2-on, Uracil, Enimycin. MW = 207: Triacsin C, Triacsin D, Streptazolin, 2-(2-Hydroxypropionyl)acetanilide, Aeruginol, 7-Deoxyechinosporin. MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.
366 nm 2 bands
AFD4 254 nm 3 bands 2 purple bands, 1 light blue band, 1 yellow band, 1 green band 10.23 12.22 12.60 211 [M + H]+, 209 [M-H] 146 [M + H]+ 137 [M + H]+, 135 [M-H] 210 145 136 9 mm zone of inhibition against MRSA A11;18 mm zone of inhibition against MRSA A5 MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline MW = 146: Indole-3-carbaldehyde MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.
366 nm 2 bands
AFD7 254 nm 4 bands 2 green bands, 2 yellow bands, 1 blue band 3.83 4.50 5.77 7.10 10.24 11.07 11.38 11.91 13.16 15.05 16.72 153 [M + H]+, 151 [M-H] 202 [M + H]+ 261 [M + H]+, 259 [M-H] 115 [M + H]+, 113 [M-H] 211 [M + H]+ 145 [M + H]+, 143 [M-H] 424 [M + H]+, 422 [M-H] 407 [M + H]+, 405 [M-H] 494 [M + H]+ 231 [M + H]+, 229 [M-H] 390 [M + H]+, 388 [M-H] 223 [M + H]+, 245 [M + Na]+ 152 201 260 114 210 144 423 406 493 230 389 222 20 mm zone of inhibition against MRSA A11; 25 mm zone of inhibition against MRSA A7 MW = 152: Guanazolo, Orsellin-aldehyde, MY3–469, Xanthine. MW = 260: Cyclo(L-Tyr-L-Pro), VibrindoleA MW = 114: 4-Hydroxy-5-methyl-furan-3-one MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline MW = 144: Pentenomycin I, 4-Epipentenomycin I MW = 423: Fibrostatin B, A9145 A, 6-Amino-9 [(aminocarbonyl)amino]-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9- hexadeoxy-decafuranuronamide MW = 389: Hatomamicin, SF-2809-I
366 nm 4 bands
AFD8 254 nm 4 bands 4 blue bands 9.07 13.42 14.94 15.58 153 [M + H]+, 151 [M-H] 257 [M + H]+, 255 [M-H] 225 [M + H]+ 181 [M + H]+ 152 256 224 180 12 mm zone of inhibition against MRSA A12;17 mm zone of inhibition against MRSA A1 MW = 152: o Hydroxyphenylacetic acid, BE-39907 A2 MW = 256: Phenacein, Albonoursin, N-Isobutyrylpyrrothine, 2,9-Dihydroxyphenazine-1-carboxylic acid
366 nm 3 bands
AFD10 254 nm 4 bands 3 yellow bands, 2 brown bands 9.90 12.60 15.61 19.42 211 [M + H]+ 137 [M + H]+,135 [M-H] 401 [M + H]+, 399 [M-H] 475 [M + H]+, 473 [M-H] 210 136 400 474 17 mm zone of inhibition against MRSA A7; 20 mm zone of inhibition against MRSA A2 MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline. MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C. MW = 400: Daunomycinol, Furaquinocin G, Daidzein-7-α-l-rhamnoside, Oxopropaline-E, 2 11-A. MW = 474: N-Ethyloxytetracycline, KS-619-1.
366 nm 1 band
AFD12 254 nm 3 bands 1 dark yellow band, 1 pink band, 1 green band 3.70 4.36 6.43 12.50 16.06 - 160 [M + H]+,158 [M-H] 110 [M-H] 205 [M-H] 137 [M + H]+, 135 [M-H] 211 [M + H]+ - 159 111 206 136 210 11 mm zone of inhibition against MRSA A7; 15 mm zone of inhibition against MRSA A5 MW = 159: 4-(Hydroxymethyl)quinolone, Quinazolinamine MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C. MW = 210: Nikkomycin E, (4R)-Thiolactomycin, Cyanomycin, 2-Amino-4-hydroxy-3-methyl-4-(3-pyridyl)butanoic acid.
366 nm 2 bands
AFD26 254 nm 4 bands 1 brown bands, 1 purple band, 1 light blue band, 1 greenish blue band .48 6.32 6.38 9.49 10.85 12.23 17.43 1052 [M + H]+?? 113 [M + H]+, 111 [M-H] 164 [M + H]+, 162 [M-H] 164 [M + H]+, 162 [M-H] 164 [M + H]+, 162 [M-H] 317 [M + H]+ 391 [M + H]+ 112 163 163 163 316 390 10 mm zone of inhibition against MRSA A1; 15 mm zone of inhibition against MRSA A7 MW = 112: Emimycin, 3,5-Dimethyl-3-oxol-2-on, Uracil, Enimycin. MW = 163: L-β-(3-Hydroxyureido)-alanine, Homoalanosine, β-Phenylethylacetamide, 2-N-Hydroxy-3,4-dihydroisoquinoline-1-one, Streptazone D, 2-Amino-4-hydroxy-pterin, 6-Hydroxy-isatin, Dihydroabikoviromycin, 3-Nitro-1H-indazole, Kitasatodine MW = 316: 17927-D, Boxazomycin A, 1 Isobutyroxymethyl cyclohex-1(6)-ene-2,3,4,5-tetrol-2-isobutyrate. MW = 390: SF-2330, Arizonin C3.
366 nm 2 bands
  1. For details, see Supplementary Information (Figure S3-S20)