Table 5 HPLC-MS analyses of the active desert actinobacterial strain

AFD2

254 nm

4 bands

2green,

1purple,

2yellow

6.38

11.03

12.57

113 [M + H]⁺, 111[M-H]⁻

208 [M + H]⁺, 206 [M-H]⁻

137 [M + H]⁺, 135 [M-H]⁻

112

207

136

17 mm zone of inhibition against MRSA A5;32 mm zone of inhibition against MRSA A11

MW = 112: Emimycin, 3,5-Dimethyl-3-oxol-2-on, Uracil, Enimycin.

MW = 207: Triacsin C, Triacsin D, Streptazolin, 2-(2-Hydroxypropionyl)acetanilide, Aeruginol, 7-Deoxyechinosporin.

MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.

366 nm

2 bands

AFD4

254 nm

3 bands

2 purple bands, 1 light blue band, 1 yellow band, 1 green band

10.23

12.22

12.60

211 [M + H]⁺, 209 [M-H]⁻

146 [M + H]⁺

137 [M + H]⁺, 135 [M-H]⁻

210

145

136

9 mm zone of inhibition against MRSA A11;18 mm zone of inhibition against MRSA A5

MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline

MW = 146: Indole-3-carbaldehyde

MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.

366 nm

2 bands

AFD7

254 nm

4 bands

2 green bands, 2 yellow bands, 1 blue band

3.83

4.50

5.77

7.10

10.24

11.07

11.38

11.91

13.16

15.05

16.72

153 [M + H]⁺, 151 [M-H]⁻

202 [M + H]⁺

261 [M + H]⁺, 259 [M-H]⁻

115 [M + H]⁺, 113 [M-H]⁻

211 [M + H]⁺

145 [M + H]⁺, 143 [M-H]⁻

424 [M + H]⁺, 422 [M-H]⁻

407 [M + H]⁺, 405 [M-H]⁻

494 [M + H]⁺

231 [M + H]⁺, 229 [M-H]⁻

390 [M + H]⁺, 388 [M-H]⁻

223 [M + H]⁺, 245 [M + Na]⁺

152

201

260

114

210

144

423

406

493

230

389

222

20 mm zone of inhibition against MRSA A11; 25 mm zone of inhibition against MRSA A7

MW = 152: Guanazolo, Orsellin-aldehyde, MY3–469, Xanthine.

MW = 260: Cyclo(L-Tyr-L-Pro), VibrindoleA

MW = 114: 4-Hydroxy-5-methyl-furan-3-one

MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline

MW = 144: Pentenomycin I, 4-Epipentenomycin I

MW = 423: Fibrostatin B, A9145 A, 6-Amino-9 [(aminocarbonyl)amino]-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9- hexadeoxy-decafuranuronamide

MW = 389: Hatomamicin, SF-2809-I

366 nm

4 bands

AFD8

254 nm

4 bands

4 blue bands

9.07

13.42

14.94

15.58

153 [M + H]⁺, 151 [M-H]⁻

257 [M + H]⁺, 255 [M-H]⁻

225 [M + H]⁺

181 [M + H]⁺

152

256

224

180

12 mm zone of inhibition against MRSA A12;17 mm zone of inhibition against MRSA A1

MW = 152: o Hydroxyphenylacetic acid, BE-39907 A2

MW = 256: Phenacein, Albonoursin, N-Isobutyrylpyrrothine, 2,9-Dihydroxyphenazine-1-carboxylic acid

366 nm

3 bands

AFD10

254 nm

4 bands

3 yellow bands, 2 brown bands

9.90

12.60

15.61

19.42

211 [M + H]⁺

137 [M + H]⁺,135 [M-H]⁻

401 [M + H]⁺, 399 [M-H]⁻

475 [M + H]⁺, 473 [M-H]⁻

210

136

400

474

17 mm zone of inhibition against MRSA A7; 20 mm zone of inhibition against MRSA A2

MW = 210: 1-Methoxyphenazine, 1-Acetyl-β-carboline.

MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.

MW = 400: Daunomycinol, Furaquinocin G, Daidzein-7-α-l-rhamnoside, Oxopropaline-E, 2 11-A.

MW = 474: N-Ethyloxytetracycline, KS-619-1.

366 nm

1 band

AFD12

254 nm

3 bands

1 dark yellow band, 1 pink band, 1 green band

3.70

4.36

6.43

12.50

16.06

-

160 [M + H]⁺,158 [M-H]⁻

110 [M-H]⁻

205 [M-H]⁻

137 [M + H]⁺, 135 [M-H]⁻

211 [M + H]⁺

-

159

111

206

136

210

11 mm zone of inhibition against MRSA A7; 15 mm zone of inhibition against MRSA A5

MW = 159: 4-(Hydroxymethyl)quinolone, Quinazolinamine

MW = 136: Pyrrole-3-yl-2-propenamide, Anthranilamide, Octalactin C.

MW = 210: Nikkomycin E, (4R)-Thiolactomycin, Cyanomycin, 2-Amino-4-hydroxy-3-methyl-4-(3-pyridyl)butanoic acid.

366 nm

2 bands

AFD26

254 nm

4 bands

1 brown bands, 1 purple band, 1 light blue band, 1 greenish blue band

.48

6.32

6.38

9.49

10.85

12.23

17.43

1052 [M + H]⁺??

113 [M + H]⁺, 111 [M-H]⁻

164 [M + H]⁺, 162 [M-H]⁻

164 [M + H]⁺, 162 [M-H]⁻

164 [M + H]⁺, 162 [M-H]⁻

317 [M + H]⁺

391 [M + H]⁺

112

163

163

163

316

390

10 mm zone of inhibition against MRSA A1; 15 mm zone of inhibition against MRSA A7

MW = 112: Emimycin, 3,5-Dimethyl-3-oxol-2-on, Uracil, Enimycin.

MW = 163: L-β-(3-Hydroxyureido)-alanine, Homoalanosine, β-Phenylethylacetamide, 2-N-Hydroxy-3,4-dihydroisoquinoline-1-one, Streptazone D, 2-Amino-4-hydroxy-pterin, 6-Hydroxy-isatin, Dihydroabikoviromycin, 3-Nitro-1H-indazole, Kitasatodine

MW = 316: 17927-D, Boxazomycin A, 1 Isobutyroxymethyl cyclohex-1(6)-ene-2,3,4,5-tetrol-2-isobutyrate.

MW = 390: SF-2330, Arizonin C3.

366 nm

2 bands