Fig. 3
Accumulation of Arg+ mutations over nine days in the wild-type CV1000 strain and strains deficient in Mfd (CV1001), MutY (CV1002), or both (CV1003) when (a) no treatment, (b) induction with IPTG for two hours, (c) treatment with 1 mM t-BHP for two hours, or (d) induction with IPTG and treatment with 1 mM t-BHP for two hours. The line on each section is at the 5 revertants per 107 CFU for easier comparison. Means are shown for each strain. The error bars represent standard error. Means were compared using the SPSS software package and one-way ANOVA. To establish whether two means were significantly different, we used the least significant differnce (LSD) test (P < 0.05) between SPSS package. Lower case letters were used to denote significant differences between means. “a”, “b”, and “c” are significantly different mean groups. ANOVA and and LSD tests were conducted separately for each day after plating. These experiments were replicated three times, and each replicate experiment comprise of five repetitions. The total number of observations is 15