Table 1 Technical settings of the CASA system SpermVision™ for motility analysis
From: Interaction of different Chlamydiae species with bovine spermatozoa
Parameter | Setting |
|---|---|
Field-of-view depth = Depth of sample chamber | 20 μm |
Light adjustment | 90–105 |
Total number of cells evaluated or number of fields | 4000 spermatozoa or 8 fields |
Sperm recognition area | 22–99 μm2 |
Frame rate | 60 frames/sec. |
Points assessed for sperm motility | 11 |
Total motility | progressive motility + local motility |
Immotile sperm | AOC < 5° |
Local motility | DSL < 6.0 μm |
Progressive motility | Every cell that is not “immotile” or “local motile” |
Hyperactive sperm | VCL > 80 μm/s, ALH > 6.5 μm and LIN < 0.65 |
Linear sperm | STR > 0.5 and LIN > 0.35 |
Non-linear sperm | STR ≤ 0.5 and LIN ≤ 0.35 |
Curvilinear sperm | DAP/Radius ≥ 3 and LIN < 0.5 |