Functional characterization and proteomic analysis of lolA in Xanthomonas campestris pv. campestris

Liao, Chao-Tsai; Chiang, Ying-Chuan; Hsiao, Yi-Min

doi:10.1186/s12866-019-1387-9

BMC Microbiology

Table 1 Putative lipoproteins, TonB-dependent receptor family, and virulence- and biofilm-related proteins in which expression was differentially altered after lolA mutation^a

From: Functional characterization and proteomic analysis of lolA in Xanthomonas campestris pv. campestris

Gene ID^b	Protein name	Cellular component	Biological process	Molecular function	Fold change^c (H27/Xcc17)	RT-qPCR^d (H27/Xcc17)
Putative lipoprotein with signal peptidase II cleavage site
XC_0253	Dipeptidyl anminopeptidase			serine-type peptidase activity	†	0.58
XC_1519	Alkaline phosphatase			phosphatase activity	0.40	0.69
XC_2148	Metallopeptidase			metalloendopeptidase activity	‡	1.41
XC_3476	Uncharacterized protein				†	0.39
XC_4152	Cytochrome C biogenesis protein	cell	cell redox homeostasis	antioxidant activity oxidoreductase activity	†	0.30
TonB-dependent receptor family
XC_0124	TonB-dependent receptor	cell outer membrane	transport	receptor activity	†	0.42
XC_0687	TonB-dependent receptor	cell outer membrane	transport	receptor activity	0.34	0.31
XC_0806♦	TonB-dependent receptor	cell outer membrane	transport	receptor activity	†	0.40
XC_1546	TonB-dependent receptor	cell outer membrane integral component of membrane	transport	receptor activity	0.26	0.35
XC_1644	TonB-dependent receptor	cell outer membrane integral component of membrane	transport	receptor activity	2.30	0.59
XC_2194	TonB-dependent receptor	cell outer membrane	transport	receptor activity	†	0.09
XC_2899	TonB-dependent receptor	cell outer membrane	transport	receptor activity	†	0.51
XC_3063	TonB-dependent receptor	cell outer membrane	transport	receptor activity	0.31	0.38
XC_0558	Ferric pseudobactin M114 receptor protein	cell outer membrane	siderophore transport	iron ion binding receptor activity	0.49	0.33
XC_3559▴	Putative siderophore receptor	cell outer membrane integral component of membrane	siderophore transport	iron ion binding receptor activity	†	0.80
Virulence-related protein
XC_0626•	Glucanase		cellulose catabolic process	hydrolase activity, hydrolyzing O-glycosyl compounds	†	0.61
Gene ID^b	Protein name	Cellular component	Biological process	Molecular function	Fold change^c (H27/Xcc17)	RT-qPCR (H27/Xcc17)
XC_0639♦	Endoglucanase		cellulose catabolic process	carbohydrate binding cellulase activity	0.42	0.62^#
XC_1632♦	VirB8 protein	integral component of membrane		oxidoreductase activity	†	0.52
XC_1806•	Virulence regulator	intracellular	regulation of transcription, DNA-templated	DNA binding	0.49	0.83
XC_3540•	Uncharacterized protein				0.43	0.0.57
XC_3696♦	Uncharacterized protein				†	0.27
Biofilm-related protein
XC_1921	Uncharacterized protein				†	0.78
XC_3686	Uncharacterized protein	integral component of membrane			†	0.87
XC_4290	Hemagglutinin	integral component of membrane	cell communication	calcium ion binding	0.32	0.20^#

^a: Detailed information is provided in Additional file 2: Table S3.
^b: Gene ID is based on X. campestris pv. campestris strain 8004. ♦, ▴, and • indicate proteins have been reported previously to be virulence-related in X. campestris pv. campestris, X. oryzae pv. oryzicola, and X. oryzae pv. oryzae, respectively.
^c: † and ‡ indicate the protein is unique to the wild type Xcc17 and the lolA mutant H27, respectively.
^d: ^# indicates the previously tested genes presented Fig. 4.

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ISSN: 1471-2180

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