Fig. 1

Gelelctrophoresis of nested PCR products of species specific primers of Leishmania donovani. M – molecular marker (100 bp), Lanes 1 to 4 – First PCR products, Lanes 5 to 8 – Second PCR products, Lanes 1 and 5 – Positive control, Lanes 2,4,6 & 8 – Negative controls, Lanes 3 and 7 – Test sample. Expected fragment size for the 1st PCR was 576 bp and 2nd PCR was 378 bp