Fig. 2

Images of RAW 264.7 macrophages treated with CCL2 chemokine and infected by L. interrogans, analyzed by confocal microscopy 1 h or 2 h after infection. a and c) Control cells, non-treated and non-infected; e and g) Cells CCL2-treated and non infected; i and k) Cells CCL2 non-treated and infected; m and o) Cells CCL2-treated and infected. L. interrogans detected in green by antibodies FITC-conjugated. Nucleus of cells stained in blue by DAPI marker, cell’s cytoplasm stained in red by Evans blue. Amplifications of slides (b, d, f, h, j, l, n and p) are indicated in white squares