MiR-3470b promotes bovine ephemeral fever virus replication via directly targeting mitochondrial antiviral signaling protein (MAVS) in baby hamster Syrian kidney cells

Table 1 The sequences of primers used for construction of MAVS recombinant vector

Gene	Primer name	Sequences (5′-3′)	Size(bp)
MAVS	MAVS-EcoR I-F	CCGGAATTCGCCACCATGGACTACAAGGACGACGATGACAAGACATTCGCTGAGGACAAG	1497
MAVS	MAVS-Not I-R	AAGGAAAAAAGCGGCCGCTCATTGGGCCAGGTGCCTACTACGGTAC	1497
MAVS 3’UTR WT	Pme I-F	AGCTTTGTTTAAACAGCCTCAGCTGCGTGCCGTTCGCT	657
MAVS 3’UTR WT	XbaI-R	CTAGTCTAGAGAGGTGGGGTTAGGAGGCTGGAGCA	657
MAVS 3’UTR Mut	Pme I-F	AGCTTTGTTTAAACAGCCTCAGCTGCGTGCCGTTCGCT	481
	R1	GGAACCAGCGTGAGACCTGCTCCCAGGCTCTAGTA	481
	F1	CAGGTCTCACGCTGGTTCCTAGGGAGCTTTG	177
	XbaI-R	CTAGTCTAGAGAGGTGGGGTTAGGAGGCTGGAGCA	177

Note: Underlined and italic parts of primers reprensent restriction endonucleases sites and flag-tagged sequences, respectively

ISSN: 1471-2180