Fig. 2
MiR-3470b promotes BEFV replication in BHK-21 cells. a BHK-21 cells were transfected with 50 nM miR-3470b mimic, 50 nM miR-3470b inhibitor and 50 nM irrelevant-targeting negative control groups (miR-NC mimic and miR-NC inhibitor) for 36 h respectively. The efficiency for transfection of the miR-3470b mimics and miR-3470b inhibitor were detected by SYBR Green based RT-qPCR assay. b BEFV genome N mRNA levels were determined by relative quantitative RT-PCR assay and fold changes were calculated using the 2-ΔΔCt method. All data were representative of three independent experiments and presented as means ± SD. ***p < 0.001, **p < 0.01, and *p < 0.05. c BEFV G protein levels were detected by western blot with anti-G polyclone antibody. Numbers below the image was used for quantifications of protein blot intensities by gray value analysis derived from image J software. d BHK-21 cells were inoculated with 0.1MOI BEFV after transfection with 50 nM of miR-3470b mimic, miR-NC mimic, miR-3470b inhibitor and miR-NC inhibitor respectively for 24 h. BEFV titers were determined by TCID50 with the Reed-Muench endpoint method