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Table 2 Sensitivity testing of the real-time and LFD-RPA with a dilution series of plasmid DNA in comparison with real-time PCR results

From: Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay

Template copy number Real-time PCR Real-time RPA LFD-RPA
Ct Value Pos. reactions/No. of runs Means Tt value (min) Pos. reactions/No. of runs
50,000 20.2 5/5 3.2 5/5
5000 24.1 5/5 5.2 5/5
500 27.7 5/5 5.4 5/5
50 30.8 5/5 5.5 5/5
5 36.1 2/5 6.2 5/5
Neg. control N.D. 0/5 N.D. 0/5
  1. Ct cycle threshold, Tt time threshold, Neg. Negative, Pos. Positive, N.D. None determined