Table 2 Sensitivity testing of the real-time and LFD-RPA with a dilution series of plasmid DNA in comparison with real-time PCR results
From: Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay
Template copy number | Real-time PCR | Real-time RPA | LFD-RPA | |
|---|---|---|---|---|
Ct Value | Pos. reactions/No. of runs | Means Tt value (min) | Pos. reactions/No. of runs | |
50,000 | 20.2 | 5/5 | 3.2 | 5/5 |
5000 | 24.1 | 5/5 | 5.2 | 5/5 |
500 | 27.7 | 5/5 | 5.4 | 5/5 |
50 | 30.8 | 5/5 | 5.5 | 5/5 |
5 | 36.1 | 2/5 | 6.2 | 5/5 |
Neg. control | N.D. | 0/5 | N.D. | 0/5 |