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Fig. 2 | BMC Microbiology

Fig. 2

From: Novel virulence, antibiotic resistance and toxin gene-specific PCR-based assays for rapid pathogenicity assessment of Arcobacter faecis and Arcobacter lanthieri

Fig. 2

Typical amplicons for each gene-specific mono and multiplex PCR assays developed for the detection of virulence, antibiotic resistance and toxin genes in A. lanthieri. Panel a Lane 1: cdtB (303 bp), cadF (243 bp) and irgA (186 bp); Lane 5: cdtC (196 bp) and pldA (132 bp); Panel b Lane 1: cdtA (220 bp) and mviN (170 bp); Lane 5: ciaB (207 bp) and tylA (125 bp); Panel c Lane 1: tet(O) (94 bp); Lane 5: tet(W) (197 bp); Lanes 1&5: A. lanthieri LMG 28516 served as control positive; Lanes 2-4 and 6-8: A. butzleri ATCC 49616, A. cibarius LMG 21996 A. faecis LMG 28519 and Lanes 9&10 served as control negative. Lane M: 1kb DNA marker

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