Genotyping of Coxiella burnetii in sheep and goat abortion samples

BMC Microbiology

Table 1 Amplification of 10 loci from panel A as this has been established (http://mlva.u-psud.fr//MLVAnet/spip.php?rubrique50)

ID	Host	Source	Location	Year	Ct	MLVA											MST type
						Loci targeted										Type
						ms01	ms03	ms07	ms12	ms20	ms21	ms22	ms26	ms30	ms36	Type
27	Sheep	Stomach content	Grevena	2015	27.1	0	0	0	-2	3	0	−7	N/A	0	3	A
45/2	Sheep	Stomach content	Elassona	2015	28.6	0	0	0	−2	3	0	−7	N/A	0	3	A
85/1	Sheep	Cotyledon	Kalavryta	2014	29.9	0	0	0	−2	3	0	−7	N/A	0	3	A
85/1	Sheep	Stomach content	Kalavryta	2014	27.4	0	0	0	−2	3	0	−7	N/A	0	3	A
92	Sheep	Liver	Edessa	2015	28.3	0	0	0	−2	3	0	−7	N/A	0	3	A
113	Sheep	Stomach content	Thessalonika	2015	23.5	0	0	0	−2	3	0	−4	N/A	0	3	B	32
152/2	Sheep	Cotyledon	Patra	2016	29.1	0	0	0	−2	3	0	−7	N/A	0	3	A
156/B	Sheep	Stomach content	Aridea	2016	28.4	0	0	0	−2	3	0	−7	N/A	0	3	A
173	Sheep	Liver	Elassona	2016	24.6	0	0	0	−2	3	0	−7	N/A	0	3	A	32
173	Sheep	Stomach content	Elassona	2016	23.7	0	0	0	−2	3	0	−7	N/A	0	3	A	32
14/1	Sheep	Cotyledon	Larissa	2017	24.2	0	0	0	−2	3	0	1	N/A	−1	9	C	32

ID Sample Identification number, Ct Real-time PCR cycle threshold, N/A not determined DNA. The genotypic designation of Nine Mile (4–7–8-8-15-6-6-4-12-4, for the corresponding loci) was used to compare our findings. Where “0”, we ended up with the same band size during the MLVA typing; differences either these are “-” or “+” correspond to differences in number of repeats among our samples and Nine Mile. MLVA and MST genotypes were confirmed in C. burnetii 2014 cooperative database (http://mlva.u-psud.fr/mlvav4/genotyping/view.php) (http://ifr48.timone.univ-mrs.fr/mst/coxiella_burnetii/strains.html)

ISSN: 1471-2180