Table 4 Primers used in this study
Primer | Oligonucleotide Sequence (5′ → 3′) |
|---|---|
Protein Purification | |
 LdtP_Ext_Fw | GGGGTGATCAGATTGCGTAT |
 LdtP_Ext_Rv | ATCCTTGCGCCTCTAAAACA |
 LdtP_LIC_Fw | TTGTATTTCCAGGGCGTAGAGAAACCCATTCATGC |
 LdtP_LIC_Rv | CAAGCTTCGTCATCAGTCAGAATCTATAGGATGATCCT |
pBAD24 Cloning | |
 LdtP_NcoI_Fw | CGATTAGCCATGGTTGGATATTTAAAGATAAATAAG |
 LdtP_AccI_Rv | TTTATTTAGTCGACGATCAGTCAGAATCTATAG |
 pBAD24-LdtP_C-terminalFLAG_Fw | gatgatgataaaTGATCGTCGACCTGCAGG |
 pBAD24-LdtP_C-terminalFLAG_Rv | atctttataatcGTCAGAATCTATAGGATGATCCTC |
Sequencing | |
 T7-Promoter | TAATACGACTCACTATAGGG |
 T7-Terminator | GCTAGTTATTGCTCAGCGG |
 pBAD-Forward | ATGCCATAGCATTTTTATCC |
 pBAD-Reverse | GATTTAATCTGTATCAGG |
qRT-PCR | |
 B488_16S_Fw | CAGAACCTTACCAGCCCTTG |
 B488_16S_Rv | ATTAGCTCCGCCTCACGACT |
 B488_10900_Fw | GGTGGCCACAGGTGGTAATA |
 B488_10900_Rv | TAACCCATGTCGTGCTTGAA |