Fig. 2
From: VirB10 vaccination for protection against Anaplasma phagocytophilum
Sera from C3H/HeN immunized mice reacted against A. phagocytophilum VirB9-1, VirB9-2, and VirB10 proteins. Proteins from equal amounts (108) of host cell-free A. phagocytophilum were separated by SDS-PAGE gel electrophoresis. Immunoblots of transferred proteins were reacted with pooled sera from the 3 immunized but not challenged mice (1:1000) and reactions were visualized by chemiluminescence. The sizes of protein standards are indicated on the left in kDa. Predicted molecular weights for A. phagoytophilum VirB9-1 (30.5KDa), VirB9-2 (28.6KDa), and VirB10 (49.2KDa). Bands of similar sizes were not visualized in sera from the pcDNA3.1/Ovalbumin control group