Fig. 1
From: VirB10 vaccination for protection against Anaplasma phagocytophilum
Analysis of the recombinant proteins VirB9-1, VirB9-2 and VirB10. SDS-PAGE and Western Blot analysis of recombinant VirB9-1 (a), VirB9-2 (b), and VirB10 (c). Lanes represent the different fractions analyzed during the purification procedure, 1 = Total crude protein, 2 = Filtered supernatant fraction obtained after high-speed centrifugation, 3 = Washed fraction, 4 = Eluted protein. Western Blot analysis was performed using the monoclonal anti-histidine tag antibody reacting with the recombinant protein (red arrows). Predicted molecular weights plus tags for VirB9-1 (33.5KDa), VirB9-2 (31.6KDa) and VirB10 (52KDa)