Table 3 Production of α-Amylases by 2-Deoxy D-Glucose Resistant Mutant Derivatives of A. oryzae Grown under Submerged Conditions on Soluble Starch
Sr. # | γ-ray Exposure | α-amylase (U ml-1 ) | Protein (mg ml-1 ) | Specific activity (U mg-1 ) |
|---|---|---|---|---|
1 | Parental Koji | 6.55±0.45 | 0.21±0.011 | 31.8 |
2 | M-60(M5) | 15.49±1.05 | 0.14±0.007 | 114.5 |
3 | M-80(10) | 26.65±1.81 | 0.11±0.006 | 251.5 |
4 | M-80(11) | 9.76±0.57 | 0.14±0.007 | 70.0 |
5 | M-80(12) | 4.92±0.29 | 0.1±0.005 | 48.9 |
6 | M-100(6) | 26.77±1.39 | 0.07±0.004 | 370.7 |
7 | M-100(12) | 17.83±0.93 | 0.05±0.003 | 332.7 |
8 | M-120(5) | 26.22±1.36 | 0.12±0.006 | 215.2 |
9 | M-120(11) | 21.05±1.10 | 0.18±0.009 | 118.0 |
10 | M-140(6) | 19.47±1.01 | 0.26±0.013 | 75.7 |