Fig. 3

Scanning Electron Microscopy (SEM) images of C. albicans biofilms formed from the CYR1OE and PDE2 strains. A: a1–4: Farnesol untreated wild strain. b1–4: Farnesol untreated CYR1OE strain. c1–4: Farnesol untreated PDE2OE strain. d1–4: Farnesol treated wild strain. e1–4: Farnesol treated CYR1OE strain. f1–4: Farnesol treated PDE2OE strain. Magnification: 2000×. The surface of the cells in a1–4, d1–4 was uneven (red arrows), while the surface of cells in b1–4, c1–4, e1–4, f1–4 was smooth with spores (yellow arrows). The other results were the same as Fig. 2A. B: The biofilms formed from the CYR1OE and PDE2OE strains exposed to farnesol consisted of shorter hyphae than that without farnesol. The hyphae extended much longer in the later phases (24 and 36 h) of the biofilms than did that in the early phases (6 h). Significance was calculated using one-way ANOVA with post ad-hoc Dunnett’s multiple comparison test. *: p < 0.05. **: p < 0.01