Screening, purification and characterization of thermostable, protease resistant Bacteriocin active against methicillin resistant Staphylococcus aureus (MRSA)

Table 1 Inhibitory spectrum of BAC-IB17 against different bacterial strains

Indicator strains	Source	Temperature (°C)	Incubation time (hrs)	Sensitivity^a
Staphylococcus aureus (MRSA)	Clinical (KIBGE-IB23)	37	24	++++
Staphylococcus aureus (MSSA)	Clinical (KIBGE-IB24)	37	24	++++
Staphylococcus aureus (MSSA)	Water	37	24	++++
Staphylococcus aureus (MSSA)	Soil	37	24	++++
Staphylococcus aureus (MRSA)	Clinical Isolate	37	24	+++
Staphylococcus aureus (MRSA)	Clinical Isolate	37	24	+++
Staphylococcus aureus (MRSA)	Clinical Isolate	37	24	+++
Enterococcus faecalis	Soil	37	48	+++
Staphylococcus aureus (MRSA)	Clinical Isolate	37	24	++
Staphylococcus aureus (MRSA)	Clinical Isolate	37	24	++
Bacillus stearothermophilus	Soil (KIBGE-IB29)	60	24	++
Bacillus subtilis	Soil	37	24	++
Listeria monocytogenes	ATCC 7644	35	48	++
Staphylococcus epidermidis	Environment	37	24	++
Salmonella typhi A	Clinical Isolate	37	24	+
Staphylococcus aureus (MRSA)	Clinical Isolate	37	24	+
Salmonella typhimurium	Clinical Isolate	37	24	+
Escherichia coli	ATCC 8739	35	18	+
Bacillus licheniformis	Environment (KIBGE-IB2)	40	24	+
Bacillus licheniformis	Soil (KIBGE-IB4)	40	24	–

^aKey: (−) No Antgonistic Activity; (+) Inhibitory zone diameter within 11-15 mm; (++) Inhibitory zone diameter within 16-20 mm; (+++) Inhibitory zone diameter within 21-25 mm; (++++) Inhibitory zone diameter within 26-30 mm

ISSN: 1471-2180