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Table 3 C5b-9 assay variability for strains 3655, 3224A and MPJ003 (14 sera per strain in triplicate, n = 126)

From: Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzae

  Description Sera with defined CV Mean CV
(±SEM)
< 20% < 35% < 40% > 40%
Intra-assay variability
n = 126
3 plates,
1 operator,
1 day
37 3 0 2 12.69
(1.84)
Inter-assay variability
n = 126
1 plate,
1 operator,
3 days
30 9 1 2 15.87
(1.71)
Inter-operator variability
n = 126
1 plate,
3 operators,
1 day
26 11 0 5 20.27
(2.60)