Development of flow cytometric opsonophagocytosis and antibody-mediated complement deposition assays for non-typeable Haemophilus influenzae

Table 3 C5b-9 assay variability for strains 3655, 3224A and MPJ003 (14 sera per strain in triplicate, n = 126)

	Description	Sera with defined CV				Mean CV (±SEM)
	Description	< 20%	< 35%	< 40%	> 40%	Mean CV (±SEM)
Intra-assay variability n = 126	3 plates, 1 operator, 1 day	37	3	0	2	12.69 (1.84)
Inter-assay variability n = 126	1 plate, 1 operator, 3 days	30	9	1	2	15.87 (1.71)
Inter-operator variability n = 126	1 plate, 3 operators, 1 day	26	11	0	5	20.27 (2.60)

ISSN: 1471-2180