Fig. 3
From: The culture of primary duck endothelial cells for the study of avian influenza
Duck endothelial cells can be isolated from the aorta of embryonated eggs. Representative immunofluorescence images and FACS plots of duck aortic endothelial cells (a) and chicken aortic endothelial cells (b) following a 4 hour incubation with Alexa Flour®488 conjugated Ac-LDL. Duck and chicken aortic endothelial cells were passaged 15 times and 17 times respectively in EGMTM-2MV medium. EA-hy926 and NCl-H441 cells were used as positive and negative control respectively for the uptake of Ac-LDL. Scale bar: 100 nm