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Table 3 Analysis of ITS1 sequence length, microsatellite markers and Wolbachia status in Glossina laboratory populations

From: Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species

Glossina species Country of origin (Location) No ITS1 expected size Wolbachia Microsatellites Correctly identified samples
cytoplasmic chromosomal A10 Gmm14
G. pallidipes IPCL
Uganda
8 920 0.0 % (0/8) 0.0 % (0/8) - + 8/8
IPCL
Ethiopia
8 12.5 % (1/8) 0.0 % (0/8) - + 8/8
G. m. morsitans IPCL 8 775 75 % (6/8) 100 % (8/8) - + 8/8
G. m. centralis IPCL 8 ~800 + ~150 100 % (8/8) 0.0 % (0/8) - + 8/8
G. m. submorsitans CIRDES 12 ~800 + ~150 0.0 % (0/12) 0.0 % (0/8) - + 12/12
G. p. gambiensis IPCL
POUT
8 543 0.0 % (0/8) 0.0 % (0/8) + + 8/8
IPCL
CIRDES
8 0.0 % (0/8) 0.0 % (0/8) + + 8/8
G. f. fuscipes IPCL
Central Africa Republic
8 618 12.5 % (1/8) 0.0 % (0/8) Partial + 8/8
G. brevipalpis IPCL 8 778 75 % (6/8) 0.0 % (0/8) - - 8/8
G. tachinoides CIRDES 12 597 0.0 % (0/12) 0.0 % (0/8) - + 12/12
  1. -: no amplicon detected
  2. +: the expected amplicon was detected in all individuals screened
  3. Partial: the expected amplicon was detected, but not in all individuals screened