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Fig. 6 | BMC Microbiology

Fig. 6

From: Nuclear and Wolbachia-based multimarker approach for the rapid and accurate identification of tsetse species

Fig. 6

Molecular Phylogenetic analysis of laboratory populations, published sequences, and selected samples from collections deriving from wild (b), by Maximum Likelihood method. Using a COI gene fragment. a The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura-Nei model. The tree with the highest log likelihood (-2609.6833) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 33 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. There was a total of 600 positions in the final dataset. Samples derived from laboratory populations of the present study are in black and different tsetse sequences available in the NCBI database are in blue. Musca domestica was used as outgroup. All abbreviations used in the Figures are shown in Additional file 5. b The evolutionary history was inferred by using the Maximum Likelihood method based on the Tamura-Nei model. The tree with the highest log likelihood (-2044.8169) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 49 nucleotide sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. There was a total of 362 positions in the final dataset. Musca domestica was used as outgroup. All abbreviations used in the Figures are shown in Additional file 5. Samples derived from laboratory populations of the present study are in black and samples collected from the field are in green.

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