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Table 1 List of Glossina and Drosophila strains analyzed in this study

From: High-sensitivity detection of cryptic Wolbachia in the African tsetse fly (Glossina spp.)

  Species Species group Strain/abbr. Origin/reference
Drosophila strains Drosophila simulans simulans NouméaTC [18] Wolbachia negative control
Drosophila willistoni willistoni P98 [19] Wolbachia positive control
  Wolbachia status (experimental)
wsp ARM blot FISH
Glossina strains Glossina morsitans morsitans morsitans Gmm Takáč Lab, Slovak Academy of Sciences, Bratislava, Slovakia +++ +++ +++ +++
Glossina morsitans centralis morsitans Gmc Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria +++ +++ +++ +++
Glossina swynnertoni morsitans Gsw Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria ++ ++ nd
Glossina palpalis palpalis palpalis Gpp Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria
Glossina palpalis gambiensis palpalis Gpg Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria + +
Glossina fuscipes fuscipes palpalis Gfu Insect Pest Control Laboratory FAO/IAEA, Vienna, Austria +? nd
  1. Two Drosophila strains were used as Wolbachia-positive and -negative controls (P98, NouméaTC). The table lists all Glossina strains used for experiments, including Wolbachia infection status based on PCR (Wolbachia outer surface protein gene, Wolbachia A-supergroup repeat motif,); blot-PCR using a wsp probe (listed as ‘blot’), and fluorescence in situ hybridization with Wolbachia 16-23S rRNA probe. Wolbachia infection titer is indicated by ‘+’ (low), ‘++’ (intermediate), and ‘+++’ (high). Abbreviations: wsp Wolbachia outer surface protein gene, ARM Wolbachia A-supergroup repeat motif, FISH fluorescence in situ hybridization, nd not determined