Fig. 1From: An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCRPerformance of detection of S. aureus by the qPCR assay. The standard curve showed a R2 of 0.99, a slope of − 3.16, and an amplification efficiency of 100.36%. The Cq values of serially diluted cell suspensions (5.0 × 107, 5.0 × 106, 5.0 × 105, 5.0 × 104, 5.0 × 103, 5.0 × 102, 5.0 × 101), indicating a LOD of 5.0 × 101 S. aureus cells with a Cq value of 37.36Back to article page