Table 2 Oligonucleotides used in this worka
From: Architecture of divergent flagellar promoters controlled by CtrA in Rhodobacter sphaeroides
| To obtain the strain AR1 | |
| DinterB2for | TCTAGATGGAACTCCTTTCAACGAC |
| DinterI2rev | TCTAGAACGGTCGTCGTCTACGA |
| To obtain pBAD_ctrA | |
| CtrABADFwSac | GAGCTCATGAGAATACTGCTGGTGGA |
| CtrABADRvEco | GAATTCTCCAGCCCACCCTTCCCG |
| To clone the wild-type promoters | |
| flgB2p (336 bp) | |
| FlgBI5 | GAGTCTGATATCCGGGCGTGTCGGCATTG |
| FlgBI2 | GAATTCCACCCGGTCGCCCAGCGCGG |
| flgE2p (303 bp) | |
| pflgE2for | GAATTCCGGTGCGAAACAACAGACT |
| pflgE2rev | GAGCTCATTGGCCGACTGCGTGAT |
| fliF2p (331 bp) | |
| FliFL5 | GAGCTCAGACCGAGCACGGCCAGGAA |
| FliFL2 | GAATTCAGGCCCGACCAGGTGGCGTAG |
| fliI2p (product of 336 bp) | |
| FliBI3 | GAATTCGATATCCGGGCGTGTCGGCATTG |
| FliBI6 | GAGCTCCACCCGGTCGCCCAGCGCGG |
| fliL2p (331 bp) | |
| FliFL3 | GAATTCAGACCGAGCACGGCCAGGAAC |
| FliFL6 | GAGCTCAGGCCCGACCAGGTGGCGTAG |
| For the 5’ RACE experiments | |
| flgB2 RACE | CGCACCATCTCATCCTCGAGCGAG |
| N3’ flgB2 RACE | GACCGTGTTGCCGTTGGGCGAAG |
| flgE2 RACE | GATATCCAGGGCGCTCGCGGTCGAGA |
| N3’ flgE2 RACE | CATTCCTCCACCCGCCGTATGGTGTT |
| fliF2 RACE | CACCTCGTAGGCCGCGCCCTGCGCC |
| fliI2 RACE | GGGCAGGATCGCCACCTCGTGCGACGAA |
| N3’ fliI2 RACE | GCCGCAGAACCTCGCCGCCGAGGATG |
| fliL2 RACE | CCAAGGCTGATCACGATCGGGTCGA |
| N3’ fliL2 RACE | CGATCGGCACGAAGGCGATGTCGGGA |
| For site directed mutagenesis | |
| flgB2p −10 | GTTTCACCAAGGCGCAAGGGCGATTCCTTTAG |
| flgB2p −13/−14 | CACCAAGGCTTCGGGGCGATTC |
| flgB2p −17/−18 | CAAGGCTTAAGGCGGATTCCTTTAGAAAG |
| flgB2p −23/−24 | CTTAAGGGCGATTGGTTTAGAAAGGGTAAG |
| flgB2p −27/−28 | GGGCGATTCCTTCGGAAAGGGTAAGGCGC |
| flgB2p −35 | TAGAAAGGGTGGGGCGCGAAC |
| flgB2p −42/−43 | GAAAGGGTAAGGCGGCAACAAAGAGGGATTTG |
| fliF2p −10 | CCACATCCGTCCCGGATGGTCGGGC |
| fliF2p −35 | GATTGTTGGGCCACATCCGTCA |
| fliI2p −10 | TTGTTCGCGCCTCCCCCTTTCT |
| fliI2p −26/−27 | TTAAGGGCGAGGCCTTTAGAAAG |
| fliI2p −35 | CACCAAGGCTTCGGGGCGATTC |
| fliI2p −47/−48 | CTCCCTCGATCGGTGCCACCAAGGCTTAAGGGC |
| fliL2p −10 | GTCGATCTAGGGGCCGGATCCC |
| fliL2p −35 | GATTGTTGGGCCACATCCGTCA |