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Table 2 Oligonucleotides used in this worka

From: Architecture of divergent flagellar promoters controlled by CtrA in Rhodobacter sphaeroides

To obtain the strain AR1

 DinterB2for

TCTAGATGGAACTCCTTTCAACGAC

 DinterI2rev

TCTAGAACGGTCGTCGTCTACGA

To obtain pBAD_ctrA

 CtrABADFwSac

GAGCTCATGAGAATACTGCTGGTGGA

 CtrABADRvEco

GAATTCTCCAGCCCACCCTTCCCG

To clone the wild-type promoters

 flgB2p (336 bp)

  FlgBI5

GAGTCTGATATCCGGGCGTGTCGGCATTG

  FlgBI2

GAATTCCACCCGGTCGCCCAGCGCGG

 flgE2p (303 bp)

  pflgE2for

GAATTCCGGTGCGAAACAACAGACT

  pflgE2rev

GAGCTCATTGGCCGACTGCGTGAT

 fliF2p (331 bp)

  FliFL5

GAGCTCAGACCGAGCACGGCCAGGAA

  FliFL2

GAATTCAGGCCCGACCAGGTGGCGTAG

 fliI2p (product of 336 bp)

  FliBI3

GAATTCGATATCCGGGCGTGTCGGCATTG

  FliBI6

GAGCTCCACCCGGTCGCCCAGCGCGG

 fliL2p (331 bp)

  FliFL3

GAATTCAGACCGAGCACGGCCAGGAAC

  FliFL6

GAGCTCAGGCCCGACCAGGTGGCGTAG

For the 5’ RACE experiments

 flgB2 RACE

CGCACCATCTCATCCTCGAGCGAG

 N3’ flgB2 RACE

GACCGTGTTGCCGTTGGGCGAAG

 flgE2 RACE

GATATCCAGGGCGCTCGCGGTCGAGA

 N3’ flgE2 RACE

CATTCCTCCACCCGCCGTATGGTGTT

 fliF2 RACE

CACCTCGTAGGCCGCGCCCTGCGCC

 fliI2 RACE

GGGCAGGATCGCCACCTCGTGCGACGAA

 N3’ fliI2 RACE

GCCGCAGAACCTCGCCGCCGAGGATG

 fliL2 RACE

CCAAGGCTGATCACGATCGGGTCGA

 N3’ fliL2 RACE

CGATCGGCACGAAGGCGATGTCGGGA

For site directed mutagenesis

 flgB2p −10

GTTTCACCAAGGCGCAAGGGCGATTCCTTTAG

 flgB2p −13/−14

CACCAAGGCTTCGGGGCGATTC

 flgB2p −17/−18

CAAGGCTTAAGGCGGATTCCTTTAGAAAG

 flgB2p −23/−24

CTTAAGGGCGATTGGTTTAGAAAGGGTAAG

 flgB2p −27/−28

GGGCGATTCCTTCGGAAAGGGTAAGGCGC

 flgB2p −35

TAGAAAGGGTGGGGCGCGAAC

 flgB2p −42/−43

GAAAGGGTAAGGCGGCAACAAAGAGGGATTTG

 fliF2p −10

CCACATCCGTCCCGGATGGTCGGGC

 fliF2p −35

GATTGTTGGGCCACATCCGTCA

 fliI2p −10

TTGTTCGCGCCTCCCCCTTTCT

 fliI2p −26/−27

TTAAGGGCGAGGCCTTTAGAAAG

 fliI2p −35

CACCAAGGCTTCGGGGCGATTC

 fliI2p −47/−48

CTCCCTCGATCGGTGCCACCAAGGCTTAAGGGC

 fliL2p −10

GTCGATCTAGGGGCCGGATCCC

 fliL2p −35

GATTGTTGGGCCACATCCGTCA

  1. aThe undelines bases correspond to the changes introduced in the wild type sequence
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BMC Microbiology

ISSN: 1471-2180

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