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Table 1 Detection of real samples by traditional colony counting and MNP-labeled dark-field counting strategy

From: Ultrasensitive and rapid count of Escherichia coli using magnetic nanoparticle probe under dark-field microscope


Colony counting method

(CFU of 10 μL of sample)

Our counting strategy

(CFU of 10 μL of sample)

Dilute soup

163 ± 16

142 ± 14


126 ± 14

112 ± 8

  1. Statistical analysis: The experimental counting results are reported as means ± SE of triplicate independent experiments. Ten fields of view were counted for each sample. Data were analyzed using the SPSS (Statistical Package for the Social Sciences) software (Version 13.0; SPSS, Inc., Chicago, IL)