Enzymes involved in the anaerobic degradation of phenol by the sulfate-reducing bacterium Desulfatiglans anilini

Table 1 Identities of putative genes involved in phenol or benzoate-degradation

Enzymes in T. aromatica or G. metallireducens ^a	Annotation from IMG in D. anilini	Gene locus (H567DRAFT_) ^b	Mol. weight (kDa)	Sequence coverage (%) ^c	Score ^d	Identities (%) ^e
ppsα	pyruvate, water dikinase	02052	70.027	49.1	323.31	42
ppsβ	pyruvate, water dikinase	02051	39.928	21.2	93.396	38
ppsγ	acetoin utilization protein AcuB	03126	25.893	35.4	202.27	25
ppcβ	phenylphosphate carboxylase beta subunit	02059	52.24	68.2	323.31	49
ppcδ	3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (KDO 8-P phosphatase)	00862	–	–	–	46
ppcα	phenylphosphate carboxylase beta subunit	03563	51.922	12.9	76.233	33
ORF 14	phenylacetate--CoA ligase	02049	50.501	9	68.567	39
ORF 8	4-hydroxy-3-polyprenylbenzoate decarboxylase	02056	–	–	–	56
BamQ^*	6-hydroxycyclohex-1-ene-1-carboxyl-CoA dehydrogenase	01120	39.75	42.1	282.42	49
BamA^*	6-ketocyclohex-1-ene-1-carbonyl-CoA hydrolase	01121	42.886	72.7	323.31	51
BamR^*	cyclohexa-1,5-diene-1-carbonyl-CoA hydratase	01122	28.001	55.7	323.31	37
BamB^*	tungsten-dependent benzoyl-CoA reductase subunit bamB	00366	75.838	46.1	323.31	80

^aAbbreviations of the enzymes involved in phenol or benzoate (*) anaerobic degradation pathways in T. aromatica or G. metallireducens (*). ^bIMG gene locus tag from the genome of D. anilini. ^cSequence coverage represents the extent of peptides obtained during MS-MS identification of respective protein in the total proteome analyses. ^dThe protein score from an MS/MS search is derived from the ions scores. Score and sequence coverage of the peptide finger print match as indicated by the MASCOT-search engine. ^eThe highest identity of protein sequence in D. anilini to that in T. aromatica or G. metallireducens. -: Not found in total proteome analyses result

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