Table 1 Primer information and standard curves of microflora for Q-PCR

Total bacteria

F: CGGYCCAGACTCCTACGGG

y = 14.354–0.2607X

[20]

R: TTACCGCGGCTGCTGGCAC

R² = 0.997 Tm = 59.5 °C

Firmicutes

F: GGAGYATGTGGTTTAATTCGAAGCA

y = 13.073–0.2985X

[21]

R: AGCTGACGACAACCATGCAC

R² = 0.998 Tm = 60.5 °C

Bacteroidetes

F: GGARCATGTGGTTTAATTCGATGAT

y = 14.390–0.2807X

[21]

R: AGCTGACGACAACCATGCAG

R² = 0.998 Tm = 58 °C

Bifidobacterium

F: TCGCGTCYGGTGTGAAAG

y = 13.837–0.2045X

[21]

R: CCACATCCAGCRTCCAC

R² = 0.998 Tm = 61.5 °C

Enterococcus spp.

F: CCCTTATTGTTAGTTGCCATCATT

y = 14.356–0.2542X

[21]

R: ACTCGTTGTACTTCCCATTGT

R² = 0.998 Tm = 52 °C

Lactobacillus spp.

F: AGCAGTAGGGAATCTTCCA

y = 14.899–0.2863X

[22]

R: CACCGCTACACATGGAG

R² = 0.999 Tm = 58 °C

A. hydrophila

F: GAAAGGTTGATGCCTAATACGTA

y = 13.903–0.2050×

R: CGTGCTGGCAACAAAGGACAG

R² = 0.991 Tm = 53.5 °C

Enterobacteriaceae

F: CATTGACGTTACCCGCAGAAGAAGC

y = 13.870–0.1927X

[23]

R: CTCTACGAGACTCAAGCTTGC

R² = 0.995 Tm = 53 °C

Streptococcus spp.

F: AGAGTTTGATCCTGGCTCAG

y = 14.086–0.2630X

[24]

R: GTTAGCCGTCCCTTTCTGG

R² = 0.996 Tm = 59.5 °C