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Table 1 Experiment setup to evaluate the efficiency of phage therapy for the treatment of G. mellonella using single phage dose, multiple phage doses and phage cocktails

From: The therapeutic potential of bacteriophages targeting gram-negative bacteria using Galleria mellonella infection model

Regimen Model Treatment/condition
Control groups
 SET 1 Control I (untreated) Larvae were injected with 20 μL of saline.
 SET 2 Control II Larvae were infected with 20 μL of bacteria (108 CFU/mL).
 SET 3 Control III Larvae were injected with 20 μL of phage lysate (104 PFU/mL).
Single Phage Dose
 SET 4 Test-1 Larvae were infected with 20 μL of bacteria and treated with 20 μL phages within 1 h.
Multiple Phage Doses
 GROUP-1 Single dose Larvae were infected with 20 μL of bacteria and treated at 0th hour using 20 μL of phages.
 GROUP-2 Double dose Larvae were infected with 20 μL of bacteria and treated with two doses (20 μL each) of phages at 0th hour and at 6th hour.
 GROUP-3 Triple dose Larvae were infected with 20 μL of bacteria and treated with three doses (20 μL each) of phages at 0th hour, at 6th hour and 12th hours.
 GROUP-4 Quadruple dose Larvae were infected with 20 μL of bacteria and treated with four doses (20 μL each) of phages at 0th hour, at 6th, 12th and 24th hours.
Cocktail of Phages
 GROUP-A Single cocktail dose Larvae were infected with 20 μL of bacterial mixture and treated at 0th hour using 20 μL of phage cocktail.
 GROUP-B Double cocktail dose Larvae were infected with 20 μL of bacterial mixture and treated at 0th hour and at 6th hour using 20 μL of phage cocktail.
 GROUP-C Triple cocktail dose Larvae were infected with 20 μL of bacterial mixture and treated at 0th hour and at 6th, 12th hour using 20 μL of phage cocktail.
 GROUP-D Quadruple cocktail dose Larvae were infected with 20 μL of bacterial mixture and treated at 0th hour and at 6th, 12th, 24th hour using 20 μL of phage cocktail.
 GROUP-E Quintuple cocktail dose Larvae were infected with 20 μL of bacterial mixture and treated at 0th hour and at 6th, 12th, 24th, 30th hour using 20 μL of phage cocktail.
  1. *In each group 10 larvae were used for the study. For all the groups, the survivability of the larvae was evaluated for 96 h and the results were interpreted statistically