Global gene-expression profiles of intracellular survival of the BruAb2_1031 gene mutated Brucella abortus in professional phagocytes, RAW 264.7 cells

Table 1 Different characteristics of RAW 264.7 cells infected with mutant strains, as compared to wild-type infected cells

Strains	Nitric oxide (μg/mL)	IL-6 (pg/mL)	TNF-α (pg/mL)	Growth rates (%)
Wild-type	0.57 ± 0.10	3.61 ± 2.64	1219.58 ± 38.91
C3	0.24 ± 0.03**	N.D.¹⁾	1183.69 ± 81.12	87.7 ± 27.9
C24	22.62 ± 0.11**	N.D.	1439.00 ± 71.73**	104.0 ± 1.1
C30	38.81 ± 0.22**	41.64 ± 8.72**	1334.62 ± 43.72*	111.4 ± 16.1

The growth rates are presented as the relative percentage compared to that of the wild-type, when growth rate of wild-type is considered as 100%. N.D.¹⁾, non-detected, the product levels of IL-6 in RAW 264.7 cells infected with C3 and C24 mutant strains were close to or below detectable levels of the ELISA system. (*p < 0.05 and **p < 0.01)

ISSN: 1471-2180