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Table 2 Assay conditions and strains for the different mAbs used for IF-FCM analysis

From: Persistent presence of outer membrane epitopes during short- and long-term starvation of five Legionella pneumophila strains

Antibody Tested strainsa antibody class Final dilution Incubation temperature Incubation time
MAb 3/1 LpParis, LpClin, LpEnv IgG 1/20 37 °C 90 min
MAb 8/4 LpParis, LpOlda IgG 1/20 37 °C 90 min
MAb 8/5 LpParis, LpClin, LpEnv, LpOlda IgM 1/20 37 °C 90 min
MAb 9/1 LpSG6 IgM 1/5 37 °C 90 min
MAb 20/1 LpClin IgG 1/5 4 °C 60 min
MAb 26/1 LpOlda IgG 1/10 37 °C 90 min
MAb 32/3 LpSG6 IgG 1/10 37 °C 90 min
Monofluo kit All strains 1/10 37 °C 60 min
Second. anti-IgG or IgM-FITC Ab   1/90 37 °C 30 min
  1. aOnly those strain-mAb combinations were investigated which showed positive signals in ELISA