Persistent presence of outer membrane epitopes during short- and long-term starvation of five Legionella pneumophila strains

Table 2 Assay conditions and strains for the different mAbs used for IF-FCM analysis

Antibody	Tested strains^a	antibody class	Final dilution	Incubation temperature	Incubation time
MAb 3/1	LpParis, LpClin, LpEnv	IgG	1/20	37 °C	90 min
MAb 8/4	LpParis, LpOlda	IgG	1/20	37 °C	90 min
MAb 8/5	LpParis, LpClin, LpEnv, LpOlda	IgM	1/20	37 °C	90 min
MAb 9/1	LpSG6	IgM	1/5	37 °C	90 min
MAb 20/1	LpClin	IgG	1/5	4 °C	60 min
MAb 26/1	LpOlda	IgG	1/10	37 °C	90 min
MAb 32/3	LpSG6	IgG	1/10	37 °C	90 min
Monofluo kit	All strains	–	1/10	37 °C	60 min
Second. anti-IgG or IgM-FITC Ab		–	1/90	37 °C	30 min

^aOnly those strain-mAb combinations were investigated which showed positive signals in ELISA

ISSN: 1471-2180