Fig. 5
From: Characterizing interactions of Leptospira interrogans with proximal renal tubule epithelial cells
Delayed of ROS production in LP-infected cells. TCMK-1 cells were infected either with LP or HP strain. a Detection of intracellular ROS. The nuclear green fluorescence reflects ROS. (b and c) Non-infected (NI) and infected cells were fixed and processed for Leptospira immunostaining and TUNEL staining (green) at 48 h pi. b Representative confocal images. Total bacteria were detected with TRITC-labeled secondary antibodies. DNA was stained with TO-PRO-3 (blue). The merged image of immunofluorescence and differential interference contrast (DIC) are also shown. c Quantification of TUNEL positive cells. Data are the mean ± SD of three independent experiments