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Table 2 Stability of Lux construct function during intramuscular infection with M89:Lux or M75::Lux

From: Modelling invasive group A streptococcal disease using bioluminescence

 

Thigh muscle

Lymph node

Spleen

Liver

Blood

M89::lux

 Number of mice with bacteria present after 24ha

6/6

1/6

2/6

3/6

0/6

 % colonies bioluminescent b(Number counted)

89% (90/110, 293/300, 55/70, 49/50, 40/60, 40/65)

100% (10/10)

100% (50/50, 50/50)

99% (43/44, 10/10, 50/50)

N/A

M75::Lux

 Number of mice with bacteria present after 24ha

6/6

3/6

2/6

2/6

4/6

 % colonies bioluminescent b (Number counted)

75% (40/80, 50/85, 39/55, 55/80, 40/60, 45/50)

0% (0/5, 0/37, 0/50)

0% (0/10, 0/30)

0% (0/20, 0/20)

0% (0/10, 0/10, 0/10, 0/20)

  1. aMice infected i.m. with either M89::Lux or M75::Lux. Samples taken 24 h after infection. Number of mice where bacteria could be cultured is indicated
  2. bStability of the Lux construct was determined by determining % colonies that demonstrated bioluminescence. Where possible at least 100 colonies were counted for each tissue and each mouse